Genomic identification and characterization of the elite strains Bradyrhizobium yuanmingense BR 3267 and Bradyrhizobium pachyrhizi BR 3262 recommended for cowpea inoculation in Brazil
| Autor: | Norma Gouvêa Rumjanek, Jakson Leite, Jerri Édson Zilli, Samuel Ribeiro Passos, Gustavo Ribeiro Xavier, Jean Luiz Simões-Araújo |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
DNA Bacterial MLSA Bradyrhizobium yuanmingense 030106 microbiology lcsh:QR1-502 Rhizobia medicine.disease_cause Microbiology Bradyrhizobium lcsh:Microbiology Vigna Evolution Molecular 03 medical and health sciences RNA Ribosomal 16S Botany medicine Symbiosis Bradyrhizobium elkanii Phylogeny biology food and beverages Genomics Agricultural Inoculants 16S ribosomal RNA biology.organism_classification 030104 developmental biology Nitrogen fixation ANI DDH Root Nodules Plant Bradyrhizobium pachyrhizi Brazil Genome Bacterial Vigna unguiculata Research Paper |
| Zdroj: | Brazilian Journal of Microbiology v.49 n.4 2018 Brazilian Journal of Microbiology Sociedade Brasileira de Microbiologia (SBM) instacron:SBM Brazilian Journal of Microbiology, Vol 49, Iss 4, Pp 703-713 Brazilian Journal of Microbiology, Volume: 49, Issue: 4, Pages: 703-713, Published: DEC 2018 |
| Popis: | The leguminous inoculation with nodule-inducing bacteria that perform biological nitrogen fixation is a good example of an “eco-friendly agricultural practice”. Bradyrhizobium strains BR 3267 and BR 3262 are recommended for cowpea ( Vigna unguiculata ) inoculation in Brazil and showed remarkable responses; nevertheless neither strain was characterized at species level, which is our goal in the present work using a polyphasic approach. The strains presented the typical phenotype of Bradyrhizobium with a slow growth and a white colony on yeast extract-mannitol medium. Strain BR 3267 was more versatile in its use of carbon sources compared to BR 3262. The fatty acid composition of BR 3267 was similar to the type strain of Bradyrhizobium yuanmingense ; while BR 3262 was similar to Bradyrhizobium elkanii and Bradyrhizobium pachyrhizi . Phylogenetic analyses based on 16S rRNA and three housekeeping genes placed both strains within the genus Bradyrhizobium : strain BR 3267 was closest to B. yuanmingense and BR 3262 to B. pachyrhizi . Genome average nucleotide identity and DNA–DNA reassociation confirmed the genomic identification of B . yuanmingense BR 3267 and B. pachyrhizi BR 3262. The nod C and nif H gene analyses showed that strains BR 3267 and BR 3262 hold divergent symbiotic genes. In summary, the results indicate that cowpea can establish effective symbiosis with divergent bradyrhizobia isolated from Brazilian soils. |
| Databáze: | OpenAIRE |
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