Cytotoxicity of V-Prep Versus Phosphoric Acid Etchant on Oral Gingival Fibroblasts
| Autor: | Victor Ghoubril, Sylvie Changotade, Didier Lutomski, Joseph Ghoubril, Carole Chakar, Maher Abboud, Louis Hardan, Naji Kharouf, Elie Khoury |
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| Přispěvatelé: | Université Saint-Joseph de Beyrouth (USJ), Unité de Recherche Biomatériaux Innovants et Interfaces (URB2i (URP_4462)), Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, Biomatériaux et Bioingénierie (BB), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), univOAK, Archive ouverte |
| Rok vydání: | 2022 |
| Předmět: |
[SDV.TOX] Life Sciences [q-bio]/Toxicology
[SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials Biomaterials oral mucosa cell viability dental acid etchants phosphoric acid V-prep [SDV.TOX]Life Sciences [q-bio]/Toxicology Biomedical Engineering [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials |
| Zdroj: | Journal of Functional Biomaterials; Volume 13; Issue 4; Pages: 266 Journal of Functional Biomaterials Journal of Functional Biomaterials, 2022, 13 (4), pp.266. ⟨10.3390/jfb13040266⟩ |
| ISSN: | 2079-4983 |
| DOI: | 10.3390/jfb13040266⟩ |
| Popis: | International audience; The most used etchant in dental daily practice is the phosphoric acid (P.A.; 37%). However, acid etchants can induce necrosis on the oral mucosa and cause the ulceration of periodontal tissue when a rubber dam is not used. V-prep is a new practical alternative, and it has satisfactory results. It is used as a preparation before the application of a resin-modified glass ionomer composite (RMGIC) to bond the orthodontic brackets. The aim of this study was to investigate the effect of the V-prep on oral gingival fibroblasts cells by comparing the cell damage and cell viability after the use of V-prep and a conventional phosphoric acid etchant with different application times and concentrations. Therefore, Gingival fibroblasts passage 6 (GFP6) was grown and treated with an acid etchant and V-prep at three different concentrations (1:1, 1:2 and 1:10) for two different application durations (30 s and 1 min). The morphological changes, cell death and cell viability were assessed. Pyknosis, karyolysis, nucleus reversible and irreversible damages and membrane destruction were observed for both of the etchants at the higher concentrations and longer application durations. Mann-Whitney Utests were used for the statistical analyses. The application of the V-prep for 30 s showed better values than the acid etchant did in the cell damage analysis and cell viability analysis (p = 0.03). V-prep at a 1:10 concentration applied for a 30 s duration can preserve the viability of gingival fibroblasts cells up to 100%. The toxicity of V-prep is equal or lower than the toxicity of the acid etchant that is commonly used in dentistry. Thus, the V-prep can be used with precautions intra-orally, and it should be applied on the enamel as a gel for 30 s only before it is rinsed and removed. |
| Databáze: | OpenAIRE |
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