Crucial role of fibroblasts in regulating epidermal morphogenesis
| Autor: | Evert N. Lamme, Maria Ponec, Abdoelwaheb El Ghalbzouri |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Keratinocytes
Histology Proliferation index Integrin alpha3 Cell Separation Integrin alpha6 Biology Pathology and Forensic Medicine chemistry.chemical_compound Dermis Cell Movement Transforming Growth Factor beta Keratin Morphogenesis medicine Humans Protein Precursors Fibroblast Involucrin Cells Cultured Platelet-Derived Growth Factor Skin Artificial chemistry.chemical_classification Transglutaminases Epidermal Growth Factor integumentary system Epidermis (botany) Integrin beta1 Integrin beta4 Cell Biology Fibroblasts Keratin-10 Immunohistochemistry Cell biology Protein Subunits medicine.anatomical_structure Epidermal Cells chemistry Immunology Keratins Keratinocyte growth factor Epidermis Keratinocyte Cell Division |
| Zdroj: | Cell and Tissue Research. 310:189-199 |
| ISSN: | 0302-766X |
| Popis: | Epidermis reconstructed on de-epidermized dermis (DED) was used to investigate whether fibroblasts can substitute growth factors needed for generation of a fully differentiated epidermis. For this purpose, a centrifugal seeding method was developed to reproducibly incorporate different fibroblast numbers into DED. Using (immuno)histochemical techniques, we could demonstrate that in the absence of fibroblasts the formed epidermis consisted only of two to three viable cell layers with a very thin stratum corneum layer. However, in the presence of fibroblasts keratinocyte proliferation and migration was stimulated and epidermal morphology markedly improved. The stimulatory effect of fibroblasts showed a biphasic character: keratinocyte proliferation increased in the initial phase but decreased in later stages of cell culture. After 3 weeks culture at the air-liquid interface, the proliferation index decreased irrespective of the number of fibroblasts present within the dermal matrix to levels observed also in native epidermis. Keratin 10 was localized in all viable suprabasal cell layers irrespective of the absence or presence of fibroblasts. Keratin 6 was downregulated with increasing numbers of fibroblasts, and keratins 16 and 17 were absent in fibroblast-populated matrices. The expression of involucrin or transglutaminase 1 showed a similar pattern as for the keratins. Irrespective of the number of fibroblasts incorporated into DED, the expression of alpha(3), alpha(6), beta(1), and beta(4) integrin subunits was upregulated. In fibroblast-free DED matrices normalization of epidermal differentiation was only achieved when the culture medium was supplemented by keratinocyte growth factor. The results of this study indicate that normalization of epidermal differentiation can be achieved using a non-contractile dermal matrix populated with fibroblasts. |
| Databáze: | OpenAIRE |
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