Liquid chromatography-mass spectrometry untargeted metabolomics reveals increased levels of tryptophan indole metabolites in urine of metabolic syndrome patients
| Autor: | Katarzyna Wrobel, Armando Gómez Ojeda, Eunice Yanez Barrientos, Ma. Eugenia Garay-Sevilla, Alma Rosa Corrales Escobosa, Kazimierz Wrobel, Moises Guerrero Esperanza |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Male Indoles Degenerative Disorder Urine Pharmacology 01 natural sciences Mass Spectrometry 03 medical and health sciences Liquid chromatography–mass spectrometry Diabetes mellitus medicine Humans Metabolomics Spectroscopy 030304 developmental biology Metabolic Syndrome Indole test Principal Component Analysis 0303 health sciences business.industry 010401 analytical chemistry Tryptophan General Medicine medicine.disease Atomic and Molecular Physics and Optics 0104 chemical sciences Cross-Sectional Studies Untargeted metabolomics Metabolome Female Metabolic syndrome business Chromatography Liquid |
| Zdroj: | European Journal of Mass Spectrometry. 26:379-387 |
| ISSN: | 1751-6838 1469-0667 |
| DOI: | 10.1177/1469066720964632 |
| Popis: | Metabolic syndrome (MetS) is a multifactor condition predisposing for diabetes, cardiovascular diseases and other degenerative disorders. Although several diagnostic criteria have been established, none of them is specific and there is a call for better pathophysiological explanation of MetS and for the discovery of molecular biomarkers. Phenotype characterization at metabolome level might be useful for both purposes. To this end, our aim was to perform comparative untargeted metabolomics of urines from MetS patients and from the control group. The study participants included 52 diagnosticated and 50 healthy individuals from Leon city in central Mexico; 23 anthropometric and clinical parameters were measured and submitted to Principal Component Analysis (PCA). The obtained PCA model allowed us for selection of 11 MetS patients and 13 control subjects, correspondingly representative for each of the two groups (clearly separated in PCA). The first morning urines from these subjects were ambulatory collected and, after methanol extraction and acidification, were submitted to capillary liquid chromatography-high resolution mass spectrometry (LC-HRMS). The obtained data were analyzed on MetaboScape® platform (Bruker Daltonics). Specifically, t-test applied to LC-HRMS data revealed several ions presenting at least 3-fold higher intensities in MetS with respect to the control samples (p |
| Databáze: | OpenAIRE |
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