Effects of conventional CPB and mini-CPB on neutrophils CD162, CD166 and CD195 expression

Autor: Zdenka Holubcova, Tomas Holubec, Drahomira Holmannova, Jiri Mandak, Pavel Kunes, Jan Krejsek, Martina Kolackova
Rok vydání: 2016
Předmět:
Fetal Proteins
Male
0301 basic medicine
Median Fluorescence Intensity
medicine.medical_specialty
Receptors
CCR5
Neutrophils
Chemokine receptor CCR5
Cell Adhesion Molecules
Neuronal
Inflammatory response
Inflammation
Post surgery
law.invention
Flow cytometry
03 medical and health sciences
0302 clinical medicine
Antigens
CD
law
Cardiopulmonary bypass
Humans
Minimally Invasive Surgical Procedures
Medicine
Radiology
Nuclear Medicine and imaging
Aged
Advanced and Specialized Nursing
Cardiopulmonary Bypass
Membrane Glycoproteins
biology
medicine.diagnostic_test
business.industry
General Medicine
Middle Aged
Cardiac surgery
surgical procedures
operative
030104 developmental biology
030220 oncology & carcinogenesis
Anesthesia
biology.protein
Female
medicine.symptom
Cardiology and Cardiovascular Medicine
business
Safety Research
Zdroj: Perfusion. 32:141-150
ISSN: 1477-111X
0267-6591
Popis: Objective: Cardiac surgery is known to trigger a systemic inflammatory response. While the use of conventional cardiopulmonary bypass (CPB) results in profound inflammation, modified mini-CPB is considered less harmful. We evaluated the impact of cardiac surgery on the expression of CD162, CD166, CD195 molecules and their association with the type of CPB used. Methods and Results: Twenty-four patients were enrolled in our study. Twelve of them were operated using conventional CPB while the other twelve patients underwent surgery with mini-CPB. Blood samples were analysed by flow cytometry. We observed a significant increase in median fluorescence intensity of CD162 and CD195 that peaked instantly after surgery and normalized to the baseline value on the 1st day post surgery, whereas CD166 was initially down-regulated and its median fluorescence intensity (MFI) value increased to the baseline in the next few days. Conclusion: We observed immediate changes in the expression of CD162, CD166, and CD195 molecules on the neutrophils after surgery in both study groups of patients. The intensity of the observed changes was significantly greater in the group of patients who underwent conventional CPB compared to patients who underwent mini-CPB cardiac surgery.
Databáze: OpenAIRE
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