Fruit and Vegetable Supplemented Diet Modulates the Pig Transcriptome and Microbiome after a Two-Week Feeding Intervention
| Autor: | Sukla Lakshman, Gloria Solano-Aguilar, Jonathan Shao, Bryan T. Vinyard, Ethiopia Beshah, Joseph F. Urban, Aleksey Molokin, Harry D. Dawson, Celine Chen, Steven G. Schroeder, Saebyeol Jang |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Lipopolysaccharides
Time Factors Swine B-Lymphocyte Subsets Physiology microbiome Biology Article immune response Transcriptome Immune system 16S rDNA Vegetables Animals TX341-641 Microbiome Gene Feces Whole blood Clostridiales Nutrition and Dietetics Blood Cells fruit and vegetable WBC transcriptome Nutrition. Foods and food supply Lachnospiraceae RNA sequencing Diet Gastrointestinal Microbiome Fruit Dietary Supplements Food Science Ruminococcaceae |
| Zdroj: | Nutrients, Vol 13, Iss 4350, p 4350 (2021) Nutrients; Volume 13; Issue 12; Pages: 4350 Nutrients |
| ISSN: | 2072-6643 |
| Popis: | A study was conducted to determine the effects of a diet supplemented with fruits and vegetables (FV) on the host whole blood cell (WBC) transcriptome and the composition and function of the intestinal microbiome. Nine six-week-old pigs were fed a pig grower diet alone or supplemented with lyophilized FV equivalent to half the daily recommended amount prescribed for humans by the Dietary Guideline for Americans (DGA) for two weeks. Host transcriptome changes in the WBC were evaluated by RNA sequencing. Isolated DNA from the fecal microbiome was used for 16S rDNA taxonomic analysis and prediction of metabolomic function. Feeding an FV-supplemented diet to pigs induced differential expression of several genes associated with an increase in B-cell development and differentiation and the regulation of cellular movement, inflammatory response, and cell-to-cell signaling. Linear discriminant analysis effect size (LEfSe) in fecal microbiome samples showed differential increases in genera from Lachnospiraceae and Ruminococcaceae families within the order Clostridiales and Erysipelotrichaceae family with a predicted reduction in rgpE-glucosyltransferase protein associated with lipopolysaccharide biosynthesis in pigs fed the FV-supplemented diet. These results suggest that feeding an FV-supplemented diet for two weeks modulated markers of cellular inflammatory and immune function in the WBC transcriptome and the composition of the intestinal microbiome by increasing the abundance of bacterial taxa that have been associated with improved intestinal health. |
| Databáze: | OpenAIRE |
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