Detection of Herplex Simplex Virus-1 and -2 in Cardiac Myxomas

Autor: Apostolos Papalois, Petros Karakitsos, Katerina Tsimaratou, Ioannis S. Pateras, Theodoros Barlogiannis, Stratigoula Sakellariou, Michalis Liontos, Vassilis G. Gorgoulis, Athanassios Kotsinas, Konstantinos Evangelou
Jazyk: angličtina
Rok vydání: 2012
Předmět:
Male
Pathology
Health
Toxicology and Mutagenesis
Herpesvirus 2
Human
lcsh:Medicine
HSL and HSV
Herpesvirus 1
Human
030204 cardiovascular system & hematology
medicine.disease_cause
Polymerase Chain Reaction
law.invention
Heart Neoplasms
0302 clinical medicine
law
Polymerase chain reaction
0303 health sciences
Histocytochemistry
General Medicine
Middle Aged
3. Good health
cardiovascular system
Molecular Medicine
Female
Polymorphism
Restriction Fragment Length
Biotechnology
Research Article
medicine.medical_specialty
Article Subject
lcsh:Biotechnology
Heart Ventricles
Biology
Virus
Statistics
Nonparametric
03 medical and health sciences
lcsh:TP248.13-248.65
Genetics
medicine
Humans
Heart Atria
Molecular Biology
030304 developmental biology
Aged
lcsh:R
Case-control study
Myxoma
Herpes Simplex
medicine.disease
Herpes simplex virus
Case-Control Studies
DNA
Viral
Etiology
Cardiac myxomas
Zdroj: Journal of Biomedicine and Biotechnology
Journal of Biomedicine and Biotechnology, Vol 2012 (2012)
ISSN: 1110-7251
1110-7243
Popis: The etiology of sporadic cardiac myxomas remains elusive. The tendency for these lesions to recur following resection, their immunopathological characteristics, along with their histological and molecular profile, may implicate the presence of an infective agent in this type of tumor. In this study, we investigated the presence of herpes simplex virus (HSV) DNA in a cohort of cardiac myxomas in a tertiary referral centre. Twenty-nine formalin-fixed paraffin-embedded (FFPE) sporadic cardiac myxomas were obtained, 17 of which were shown to be informative. These were compared to 19 macroscopically and microscopically normal heart tissue specimens. The detection of HSV-1 and -2 genomic sequences was achieved with the use of a combined nested PCR-Restriction Fragment Length Polymorphism methodology. The presence of HSV-1 and/or -2 DNA was demonstrated in 6 of 17 (35%) informative sporadic cardiac myxomas, whereas no HSV DNA was detected in normal heart tissues (P<0.01). The existence of HSV-1/2 DNA in sporadic cardiac myxomas, along with its absence from normal heart tissues, reinforces the possibility that HSV infection might be involved in the development of these lesions. Our findings raise the point of anti-HSV medication postsurgically with a potential benefit in reducing the rate of recurrences.
Databáze: OpenAIRE
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