Chitosan promotes immune responses, ameliorating total mature white blood cell numbers, but increases glutamic oxaloacetic transaminase and glutamic pyruvic transaminase, and ameliorates lactate dehydrogenase levels in leukemia mice in vivo

Autor: Shu Fen Peng, Chia‑Hui Liu, Yung Luen Shih, Ming Yang Yeh, Lung‑Yuan Wu, Hsueh Yu Chung, Wen Wen Huang, Jing Gung Chung, Jia You Liu, Hsu Feng Lu, Jason Chou
Rok vydání: 2017
Předmět:
Cytotoxicity
Immunologic
Male
0301 basic medicine
Cancer Research
glutamic pyruvic transaminase
Lymphocyte Activation
Biochemistry
immune response
Leukocyte Count
Mice
chemistry.chemical_compound
0302 clinical medicine
Immunity
Cellular
Mice
Inbred BALB C
Leukemia
biology
Alanine Transaminase
Articles
Killer Cells
Natural
medicine.anatomical_structure
Oncology
030220 oncology & carcinogenesis
Molecular Medicine
medicine.medical_specialty
Phagocytosis
Spleen
Peripheral blood mononuclear cell
03 medical and health sciences
Immune system
Adjuvants
Immunologic
Cell Line
Tumor
Internal medicine
Lactate dehydrogenase
White blood cell
Genetics
medicine
Animals
Aspartate Aminotransferases
Molecular Biology
Chitosan
L-Lactate Dehydrogenase
glutamic oxaloacetic transaminase
lactate dehydrogenase
medicine.disease
Molecular biology
030104 developmental biology
Endocrinology
Alanine transaminase
chemistry
biology.protein
Zdroj: Molecular Medicine Reports
ISSN: 1791-3004
1791-2997
DOI: 10.3892/mmr.2017.6923
Popis: The aim of the present study was to investigate the effect of chitosan (a naturally derived polymer) on the immune responses and glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and lactate dehydrogenase (LDH) levels in WEHI-3 cell-generated leukemia mice. Mice were divided into control, WEHI-3 control, acetic acid (vehicle)-treated, and 5 and 20 mg/kg chitosan-treated groups. Mice were subsequently weighed, blood was collected, and liver and spleen samples were isolated and weighed. Blood samples were measured for cell markers, the spleen underwent phagocytosis and natural killer (NK) cell activity examination, and cell proliferation was analyzed by flow cytometry. Chitosan did not significantly affect the weights of body, liver and spleen at 5 and 20 mg/kg treatment. Chitosan increased the percentage of CD3 (T cells marker), decreased the levels of CD19 (B-cell marker) and CD11b at 5 mg/kg treatment, and decreased the levels of Mac-3 at 5 and 20 mg/kg treatment. Chitosan significantly increased macrophage phagocytosis of PBMCs, but did not significantly affect macrophage phagocytosis in the peritoneal cavity. Chitosan treatment did not significantly affect the cytotoxic activity of NK cells, and also did not affect T- and B-cell proliferation. Chitosan significantly increased total white blood cell numbers, and GOT and GPT activities were both significantly increased. However, chitosan did not significantly affect LDH activity in leukemia mice. Chitosan may aid in future studies on improving immune responses in the treatment of leukemia.
Databáze: OpenAIRE
Externí odkaz: