Expression of inhibitory receptor ILT3 on neoplastic B cells is associated with lymphoid tissue involvement in chronic lymphocytic leukemia
| Autor: | Lawrence Tsao, Govind Bhagat, Su Wang, Nicole Suciu-Foca, Bachir Alobeid, Julie G. Fisher, Manuel Menes, Tetsunori Seki, Hana Lin, Adriana I. Colovai, Chuan Wang |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Histology Lymphoid Tissue T cell Chronic lymphocytic leukemia Receptors Cell Surface Biology CD5 Antigens Immunophenotyping Pathology and Forensic Medicine Immune system Antigen Predictive Value of Tests immune system diseases hemic and lymphatic diseases Biomarkers Tumor medicine Humans RNA Messenger Receptors Immunologic Antigen-presenting cell neoplasms Aged B-Lymphocytes Membrane Glycoproteins Reverse Transcriptase Polymerase Chain Reaction Cell Biology Flow Cytometry medicine.disease Leukemia Lymphocytic Chronic B-Cell Leukemia medicine.anatomical_structure Lymphatic system Chronic Disease Immunology Female CD5 Biomarkers |
| Zdroj: | Cytometry Part B: Clinical Cytometry. :354-362 |
| ISSN: | 1552-4957 1552-4949 |
| DOI: | 10.1002/cyto.b.20164 |
| Popis: | T cell responses against leukemia-associated antigens have been reported in chronic lymphocytic leukemia (CLL). However, the relentless accumulation of CLL B cells in some patients indicates that anti-tumor immune responses are inefficient. Inhibitory receptors from the Ig-like transcript (ILT) family, such as ILT3 and ILT4, are crucial to the tolerogenic activity of antigen presenting cells. In this study, we examined the expression of ILT3 on CD5+ B cells obtained from 47 patients with CLL. Using flow cytometry and RT-PCR, we found that B CLL cells from 23 of 47 patients expressed ILT3 protein and mature ILT3 mRNA. ILT3 protein and mRNA were not found in normal B cells obtained from donors without CLL. Expression of ILT4 in normal and B CLL cells showed a pattern similar to ILT3. The frequency of ILT3 positive CLL B cells was higher in patients with lymphoid tissue involvement, suggesting that ILT3 may have prognostic value in CLL. Our findings indicate that expression of ILT3 and ILT4 on CLL B cells represents a phenotypic abnormality that may play a role in tolerization of tumor-specific T cells. |
| Databáze: | OpenAIRE |
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