Resetting the site: redirecting integration of an insertion sequence in a predictable way
| Autor: | Alison B. Hickman, Orsolya Barabas, Adeline Achard, Bao Ton Hoang, Frederick Dyda, Michael Chandler, Catherine Guynet |
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| Přispěvatelé: | Laboratoire de microbiologie et génétique moléculaires (LMGM), Centre de Biologie Intégrative (CBI), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Transposable element
Base pair DNA Single-Stranded Transposases Computational biology Biology Article Transposition (music) 03 medical and health sciences Bacterial Proteins Tn10 Point Mutation Insertion Insertion sequence Base Pairing Molecular Biology Transposase ComputingMilieux_MISCELLANEOUS 030304 developmental biology Sequence (medicine) Genetics 0303 health sciences Base Sequence Helicobacter pylori Models Genetic 030306 microbiology Cell Biology DNA Transposable Elements |
| Zdroj: | Molecular Cell Molecular Cell, Elsevier, 2009, 34, pp.612-619 |
| ISSN: | 1097-2765 |
| Popis: | Target site choice is a complex and poorly understood aspect of DNA transposition despite its importance in rational transposon-mediated gene delivery. Though most transposons choose target sites essentially randomly or with some slight sequence or structural preferences, insertion sequence IS608 from Helicobacter pylori, which transposes using single-stranded DNA, always inserts just 3' of a TTAC tetranucleotide. Our results from studies on the IS608 transposition mechanism demonstrated that the transposase recognizes its target site by co-opting an internal segment of transposon DNA and utilizes it for specific recognition of the target sites through base-pairing. This suggested a way to redirect IS608 transposition to novel target sites. As we demonstrate here, we can now direct insertions in a predictable way into a variety of different chosen target sequences, both in vitro and in vivo. |
| Databáze: | OpenAIRE |
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