Amentoflavone Induces Apoptosis and Inhibits NF-ĸB-modulated Anti-apoptotic Signaling in Glioblastoma Cells
| Autor: | Song Shei Lin, Chih Sheng Huang, Yen Chung Chen, Tsu Te Liu, Yao Chen Chuang, Tsung Hsien Yen, Fei Ting Hsu, Chia Ling Hsieh |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Cell Survival Cell Apoptosis Amentoflavone General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Cell Line Tumor medicine Biflavonoids Cytochrome P-450 Enzyme Inhibitors Humans MCL1 Viability assay Pharmacology Membrane Potential Mitochondrial Caspase 8 Chemistry Caspase 3 NF-kappa B Interleukin In vitro Cell biology Blot 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Apoptosis Regulatory Proteins Glioblastoma Research Article Signal Transduction |
| Popis: | >The goal of the present study was to investigate anticancer effect of amentoflavone on glioblastoma cells in vitro. Our results demonstrated that amentoflavone not only significantly reduced cell viability, nuclear factor-ĸappa B (NF-ĸB) activation, and protein expression of cellular Fas-associated protein with death domain-like interleukin 1 beta-converting enzyme inhibitory protein (C-FLIP) and myeloid cell leukemia 1 (MCL1), but significantly triggered cell accumulation at the sub-G 1 phase, loss of mitochondrial membrane potential, and expression of active caspase-3 and -8. In order to verify the effect of NF-ĸB inhibitor on expression of anti-apoptotic proteins, we performed western blotting. We found that the of NF-ĸB inhibitor or amentoflavone markedly diminished protein levels of MCL1 and C-FLIP. Taken all together, our findings show that amentoflavone induces intrinsic and extrinsic apoptosis and inhibits NF-ĸB-modulated anti-apoptotic signaling in U-87 MG cells in vitro. |
| Databáze: | OpenAIRE |
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