Migratory properties of skin dendritic cells of the rat
| Autor: | E. van Gelderop, C. D. Richters, J.S. du Pont, M.J. Hoekstra, E. W. A. Kamperdijk |
|---|---|
| Přispěvatelé: | VU University medical center |
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Male
Cellular immunity Langerhans cell medicine.drug_class Biology Monoclonal antibody Epitope Immune system Cell Movement medicine Animals Lymph node Cells Cultured Skin Transplantation Epidermis (botany) Macrophages Histocompatibility Antigens Class I Rats Inbred Strains Dendritic Cells Dendritic cell Rats Rats Inbred ACI medicine.anatomical_structure Immunology Leukocyte Common Antigens Surgery Lymph Nodes |
| Zdroj: | Transplantation Proceedings, 29, 1745-1747. Elsevier USA Richters, C D, van Gelderop, E, du Pont, J S, Hoekstra, M J & Kamperdijk, E W A 1997, ' Migratory properties of skin dendritic cells of the rat ', Transplantation Proceedings, vol. 29, pp. 1745-1747 . https://doi.org/10.1016/S0041-1345(97)00040-7 |
| ISSN: | 0041-1345 |
| DOI: | 10.1016/S0041-1345(97)00040-7 |
| Popis: | Dendritic cells (DC), in the epidermis identified as Langerhans cells (LC), are crucial in the initiation of immune responses. After allogeneic skin transplantation, LC migrating from the graft to the draining lymph node of the recipient are supposed to play an important role in the initiation of skin rejection.' In earlier studies, we observed that during culture of rat skin explants, antigen-presenting DCs including LCs migrate “spontaneously” into the medium.2,3 To obtain more insight in the migratory properties of these in vitro migrating skin DC, we injected them subcutaneously in the hind footpads of congeneic and allogeneic recipient rats. To trace migrating cells in sections of the draining popliteal lymph node, we used the rat strain PVG-RT7b as donor rats and, as recipients, the congeneic PVG and the allogeneic ACI rats. The leukocytes of the PVG-RT7b bear an epitope that can be recognized using the monoclonal antibody His 41.4 Using this model, we compared the migration patterns of the injected cells in the congeneic lymph node with those of the allogeneic. |
| Databáze: | OpenAIRE |
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