First identification of mammalian orthoreovirus type 3 by gut virome analysis in diarrheic child in Brazil
| Autor: | Antonio Charlys da Costa, Rory J Tinker, Xutao Deng, Márcia Cristina Alves Brito Sayão Lobato, Eric Delwart, Shirley Vasconcelos Komninakis, Geovani de Oliveira Ribeiro, Rogério Togisaki das Chagas, Élcio Leal, Ester Cerdeira Sabino, Cassia Vitória de Deus Alves Soares, Adriana Luchs, Ramendra Pati Pandey, V. Samuel Raj, Roozbeh Tahmasebi, Ulisses Alves Rosa, Rafael Brustulin, Maria de Fátima Neves dos Santos Abrão, Fabiola Villanova, Flavio Augusto de Pádua Milagres |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Diarrhea Rural Population Viral metagenomics Swine 030106 microbiology Reassortment lcsh:Medicine Genome Viral Biology Virus Article 03 medical and health sciences Phylogenetics Chiroptera medicine Animals Humans Human virome lcsh:Science Mammalian orthoreovirus 3 Feces Phylogeny Multidisciplinary Phylogenetic tree Molecular medicine Geography lcsh:R Phylogenomics Infant Virology Gastroenteritis Gastrointestinal Microbiome Intestines 030104 developmental biology Viral infection Child Preschool lcsh:Q Cattle Metagenomics medicine.symptom Brazil |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-7 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Diarrhea remains one of the most common causes of deaths in children. Although many studies have investigated the prevalence of enteric pathogens around the globe some diarrheal episodes remain unexplained. It is possible that some yet-unidentified viral agents could be related to these cases of gastroenteritis. By using viral metagenomics techniques, we screened 251 fecal samples of children between 0.5 to 2.5-year-old with acute diarrhea not associated with common pathogens. These children live in rural areas and have different levels of contact with animals such as pigs, cows and bats. Here we report a complete genome of one mammalian orthoreovirus (MRV) type 3, denoted TO-151/BR, detected in a female child in the state of Tocantins (north of Brazil). Brazilian TO-151/BR strain was classified as MRV-3 based on S1 phylogeny and was closely related to porcine Asian strains. Phylogenetic analyses showed that other segments were more similar to MRV-3s of different geographic locations and hosts, including human and bats, highlighting genome reassortment and lack of host-specific barriers. This is the first report of MRV-3 in South America and a hypothesis of a silent long-term circulation of this virus in Brazil has been raised. |
| Databáze: | OpenAIRE |
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