Neuroprotective effects of pregabalin in a rat model of intracisternal facial nerve avulsion
| Autor: | Mitsuhiro Hasegawa, Shigeta Moriya, Joji Inamasu, Yuichi Hirose, Ryo Higashi, Fumihiro Imai, Hirotaka Kogame, Makoto Ito |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Stylomastoid foramen Pregabalin 02 engineering and technology 01 natural sciences Cresyl violet chemistry.chemical_compound 0103 physical sciences 0202 electrical engineering electronic engineering information engineering medicine Animals Rats Wistar 010306 general physics Spinal cord injury Facial Nerve Injuries Motor Neurons business.industry medicine.disease Facial nerve Rats Disease Models Animal Neuroprotective Agents medicine.anatomical_structure chemistry Anesthesia Neuropathic pain 020201 artificial intelligence & image processing Surgery Neurology (clinical) Neuron Brainstem business Brain Stem medicine.drug |
| Zdroj: | Journal of Neurosurgical Sciences. 61 |
| ISSN: | 1827-1855 0390-5616 |
| DOI: | 10.23736/s0390-5616.16.03263-x |
| Popis: | BACKGROUND Pregabalin (PGB), a drug used for treating neuropathic pain, has immune-modulating property that may have therapeutic implications. Suppression of microglial activation and improvement in functional recovery was observed in experimental spinal cord injury after PGB administration. An experimental study was conducted to evaluate whether PGB could afford neuroprotection in a rat model of intracisternal facial nerve avulsion. METHODS Twenty-eight male Wistar rats (250-300 g) were dichotomized into two groups: a PGB group (N.=14) and a control group (N.=14). The PGB group received a total of 4 intraperitoneal PGB injections (30 mg/kg, 15 minutes preoperatively and 4, 24, and 48 hours postoperatively), and the control group underwent intraperitoneal saline injection. Intracisternal facial nerve avulsion was created by tangential pull-out of the nerve surgically exposed at the stylomastoid foramen. In both groups, the brainstem containing the facial motor nuclei neurons was thin-sliced and stained with cresyl violet, and the number of viable neurons in the facial motor nuclei on days 14 and 28 was counted under microscope. RESULTS The total viable neuron count was significantly greater in the PGB group than in the Control group both on day 14 (271.4±14.9 vs. 196.2±22.2, P |
| Databáze: | OpenAIRE |
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