Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA
Autor: | Laurence K. Grill, Damon A. Harvey, Monto H. Kumagai, J Donson, Kathleen M. Hanley, Guy Della-Cioppa |
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Rok vydání: | 1995 |
Předmět: |
Phytoene desaturase
viruses Molecular Sequence Data Nicotiana benthamiana Genes Plant Structure-Activity Relationship chemistry.chemical_compound Phytoene Gene Expression Regulation Plant Sequence Homology Nucleic Acid Complementary DNA Gene expression Amino Acid Sequence RNA Messenger Multidisciplinary Phytoene synthase Base Sequence biology Tobamovirus fungi food and beverages RNA Oligonucleotides Antisense Plants Genetically Modified biology.organism_classification Carotenoids Molecular biology Antisense RNA chemistry Mutagenesis Site-Directed biology.protein RNA Viral Oxidoreductases Sequence Alignment Research Article |
Zdroj: | Proceedings of the National Academy of Sciences. 92:1679-1683 |
ISSN: | 1091-6490 0027-8424 |
Popis: | The carotenoid biosynthetic pathway in higher plants was manipulated by using an RNA viral vector. A cDNA encoding phytoene synthase and a partial cDNA encoding phytoene desaturase (PDS) were placed under the transcriptional control of a tobamovirus subgenomic promoter. One to two weeks after inoculation, systemically infected Nicotiana benthamiana plants were analyzed for phytoene. Leaves from transfected plants expressing phytoene synthase developed a bright orange phenotype and accumulated high levels of phytoene. Cytoplasmic inhibition of plant gene expression by viral RNA was demonstrated with an antisense RNA transcript to a partial PDS cDNA derived from tomato. The leaves of the plants transfected with the antisense PDS sequence developed a white phenotype and also accumulated high levels of phytoene. A partial cDNA to the corresponding N. benthamiana PDS gene was isolated and found to share significant homology with the tomato antisense PDS transcript. This work demonstrates that an episomal RNA viral vector can be used to deliberately manipulate a major, eukaryotic biosynthetic pathway. In addition, our results indicate that an antisense transcript generated in the cytoplasm of a plant cell can turn off endogenous gene expression. |
Databáze: | OpenAIRE |
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