Vaginal ecosystem modeling of growth patterns of anaerobic bacteria in microaerophilic conditions
| Autor: | Marc M. Baum, Lauren N. Dawson, Audrie A. Medina-Colorado, Richard B. Pyles, Kathleen L. Vincent, Elena V. Kozlova, Carrie A. Maxwell, Aaron L. Miller, Trevelyn J. Olive |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Adolescent 030106 microbiology Context (language use) Biology Microbiology Models Biological Article 03 medical and health sciences Bacteria Anaerobic Young Adult medicine Humans Ecosystem Colonization Anaerobiosis Bacteriological Techniques Lactobacillus crispatus Host (biology) Carbon Dioxide Middle Aged medicine.disease biology.organism_classification Aerobiosis Bacteria Aerobic 030104 developmental biology Infectious Diseases medicine.anatomical_structure Vagina Female Anaerobic bacteria Dysbiosis |
| Zdroj: | Anaerobe. 45 |
| ISSN: | 1095-8274 |
| Popis: | The human vagina constitutes a complex ecosystem created through relationships established between host mucosa and bacterial communities. In this ecosystem, classically defined strict bacterial aerobes and anaerobes thrive as communities in the microaerophilic environment. Levels of CO2 and O2 present in the vaginal lumen are impacted by both the ecosystem’s physiology and the behavior and health of the human host. Study of such complex relationships requires controlled and reproducible causational approaches that are not possible in the human host that, until recently, was the only place these intact bacterial communities thrived. To address this need we have utilized our ex vivo human vaginal mucosa culture system to support controlled, reproducible colonization by vaginal microbiomes (VMB) collected from healthy and symptomatic donors. Parallel vaginal epithelial cells (VEC)-VMB co-cultures were exposed to increasingly microaerophilic conditions to study the impact of CO2 concentrations upon the anaerobic bacteria associated with dysbiosis and inflammation. Our data suggest that in the context of intact VMBs, increased CO2 concentrations favored specific lactobacilli species defined as aerobes or microaerophiles when grown as monocultures. The observed community changes also led to shifts in host VEC phenotypes with significant changes in the host transcriptome, including altered expression of select molecular transporter genes. These findings support the need for additional study of the environmental changes associated with behavior and health upon the symbiotic and adversarial relationships that are formed in microbial communities present in the human vaginal ecosystem. |
| Databáze: | OpenAIRE |
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