Detection by whole genome microarrays of a spontaneous 126-gene deletion during construction of a ytfE mutant: confirmation that a ytfE mutation results in loss of repair of iron–sulfur centres in proteins damaged by oxidative or nitrosative stress
| Autor: | Lígia M. Saraiva, Jeffrey A. Cole, Marta C. Justino, Claire E. Vine |
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| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
Mutation Escherichia coli Proteins Iron Mutant Nucleic Acid Hybridization Hydrogen Peroxide Oxidative phosphorylation Biology medicine.disease_cause Microbiology Genome Molecular biology Regulon Escherichia coli medicine DNA microarray Molecular Biology Gene Sulfur Sequence Deletion |
| Zdroj: | Journal of Microbiological Methods. 81:77-79 |
| ISSN: | 0167-7012 |
| Popis: | We show that genomic hybridization allows detection of a spontaneous secondary deletion of 126 genes that occurred during construction of an Escherichia coli ytfE mutant, LMS4209, explaining some of its unexpected growth defects. We confirm that YtfE is required to repair damage to iron-sulfur centres and for hydrogen peroxide resistance. |
| Databáze: | OpenAIRE |
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