5α-Dihydrotestosterone enhances wound healing in diabetic rats
| Autor: | Rômulo Dias Novaes, Mariáurea Matias Sarandy, Neire Moura de Gouveia, Reggiani Vilela Gonçalves, Sérgio Luis Pinto da Matta, Foued Salmen Espindola, Eduardo Medeiros Damasceno, Mariella Bontempo Freitas |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Drug Compounding medicine.medical_treatment Intraperitoneal injection Silver sulfadiazine Antioxidants Collagen Type I General Biochemistry Genetics and Molecular Biology Diabetes Mellitus Experimental Protein Carbonylation Cicatrix 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Animals Mast Cells Rats Wistar General Pharmacology Toxicology and Pharmaceutics Saline Testosterone Skin Wound Healing business.industry Lanolin Dihydrotestosterone General Medicine Streptozotocin Rats Surgery Collagen Type III 030104 developmental biology Endocrinology business Wound healing medicine.drug |
| Zdroj: | Life Sciences. 152:67-75 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2016.03.019 |
| Popis: | Wound healing involves a complex interaction between the cells, extracellular matrix and oxidative response. Aims Analyze the effects of 5α-Dihydrotestosterone (5α-DTH) ointment in cutaneous wound healing by secondary intention in diabetic Wistar rats. Main methods Rats (302.23 ± 26.23 g, n = 48) were maintained in cages with food and water ad libitum in accordance with the Guiding Principles in the Use of Animal Ethics Committee. Diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg). Three skin wounds (12 mm diameter) were created on the animals' back, which were randomized into 6 groups according to the application received: VT group: Vehicle (lanolin), SA group: 0.9% saline solution, NC group: Non-diabetic, CP group: positive control (silver sulfadiazine 0001%), T1 group: Testosterone (10%), T2 group: Testosterone (20%) emulsified in lanolin. The applications were made daily within 21 days, and tissues from different wounds were removed every 7 days. Key findings Both groups treated with testosterone (T1 and T2) showed a significantly higher proportion of type I and type III collagen fibers. Superoxide dismutase levels were significantly higher on days 7 and 14 in testosterone treated groups. Protein carbonyls and MDA were lower in both groups. Significance We conclude that groups treated with 5α-DTH showed a better healing pattern with complete wound closure, and proved to have a positive effect on the morphology of the scar tissue as well as an antioxidant stimulating effect during secondhand intention skin wounds repair in diabetic rats. |
| Databáze: | OpenAIRE |
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