Crystallization of a soluble form of the rat T-cell surface glycoprotein CD4 complexed with Fab from the W3/25 monoclonal antibody
| Autor: | A F Williams, Karl Harlos, Simon J. Davis, Guy Dodson, A N Barclay, R.L. Brady |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Stereochemistry
medicine.drug_class Monoclonal antibody law.invention Immunoglobulin Fab Fragments Antigen X-Ray Diffraction Structural Biology law T-Cell Surface Glycoprotein CD4 medicine Animals Crystallization Molecular Biology chemistry.chemical_classification Antibodies Monoclonal Recombinant Proteins Rats chemistry Solubility Monoclonal CD4 Antigens Recombinant DNA Orthorhombic crystal system Glycoprotein |
| Popis: | The structure of the T-lymphocyte cell surface glycoprotein CD4 is of considerable biological and medical interest. Recombinant rat CD4 expressed in soluble form in mammalian cells and complexed with W3/25 monoclonal Fab fragments formed crystals that diffract to 3·5 A and have the orthorhombic space group P 2 1 2 1 2 or P 2 1 2 1 2 1 . The unit cell has dimensions a = 317 A, b = 161 A and c = 41·8 A and the asymmetric unit consists of two CD4 : Fab complexes. These crystals are of suitable quality for X-ray diffraction analysis. |
| Databáze: | OpenAIRE |
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