Decellularized equine carotid artery layers as matrix for regenerated neurites of spiral ganglion neurons

Autor: Ulrike Böer, Verena Scheper, Theodor Doll, Suheda Yilmaz-Bayraktar, Thomas Lenarz, Mariela C. Torrente, Michaela Kreienmeyer, Jana Schwieger
Rok vydání: 2019
Předmět:
Tunica media
Neurite
Hearing Loss
Sensorineural
guiding structures
Biomedical Engineering
decellularized matrix
Medicine (miscellaneous)
Biocompatible Materials
Bioengineering
02 engineering and technology
Biomaterials
03 medical and health sciences
0302 clinical medicine
Original Research Articles
Adventitia
otorhinolaryngologic diseases
medicine
Animals
Nerve–electrode interface
Horses
Cells
Cultured
Spiral ganglion
Tissue Engineering
Bioartificial Organs and Regenerative Medicine
Decellularization
Tissue Engineering
Tissue Scaffolds
Chemistry
Tunica Adventitia
General Medicine
Anatomy
021001 nanoscience & nanotechnology
Tunica intima
Nerve Regeneration
Rats
Carotid Arteries
Cochlear Implants
medicine.anatomical_structure
cardiovascular system
sense organs
Implant
Spiral Ganglion
0210 nano-technology
030217 neurology & neurosurgery
Zdroj: The International Journal of Artificial Organs
ISSN: 1724-6040
0391-3988
Popis: Today’s best solution in compensating for sensorineural hearing loss is the cochlear implant, which electrically stimulates the spiral ganglion neurons in the inner ear. An optimum hearing impression is not ensured due to, among other reasons, a remaining anatomical gap between the spiral ganglion neurons and the implant electrodes. The gap could be bridged via pharmacologically triggered neurite growth toward the electrodes if biomaterials for neurite guidance could be provided. For this, we investigated the suitability of decellularized tissue. We compared three different layers (tunica adventitia, tunica media, and tunica intima) of decellularized equine carotid arteries in a preliminary approach. Rat spiral ganglia explants were cultured on decellularized equine carotid artery layers and neurite sprouting was assessed quantitatively. Generally, neurite outgrowth was possible and it was most prominent on the intima (in average 83 neurites per spiral ganglia explants, followed by the adventitia (62 neurites) and the lowest growth on the media (20 neurites). Thus, decellularized equine carotid arteries showed promising effects on neurite regeneration and can be developed further as efficient biomaterials for neural implants in hearing research.
Databáze: OpenAIRE
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