Striatal interneurons in dissociated cell culture
| Autor: | Sarah C. Schock, Kheira Jolin-Dahel, P. C. Schock, Marianela Garcia-Munoz, Gordon W. Arbuthnott, William A. Staines |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Histology
Interneuron Ganglionic eminence Striatum Cell Separation Cholinergic interneurons Rats Sprague-Dawley Mice Interneurons medicine Animals Cholinergic neuron Molecular Biology Cells Cultured Original Paper biology Putamen musculoskeletal neural and ocular physiology Anatomy Cell Biology Coculture Techniques Rats Neostriatum Medical Laboratory Technology medicine.anatomical_structure nervous system biology.protein Basal ganglia Cholinergic Cell culture Calretinin Neuroscience Parvalbumin |
| Zdroj: | Histochemistry and Cell Biology |
| ISSN: | 1432-119X 0948-6143 |
| Popis: | In addition to the well-characterized direct and indirect projection neurons there are four major interneuron types in the striatum. Three contain GABA and either parvalbumin, calretinin or NOS/NPY/somatostatin. The fourth is cholinergic. It might be assumed that dissociated cell cultures of striatum (typically from embryonic day E18.5 in rat and E14.5 for mouse) contain each of these neuronal types. However, in dissociated rat striatal (caudate/putamen, CPu) cultures arguably the most important interneuron, the giant aspiny cholinergic neuron, is not present. When dissociated striatal neurons from E14.5 Sprague–Dawley rats were mixed with those from E18.5 rats, combined cultures from these two gestational periods yielded surviving cholinergic interneurons and representative populations of the other interneuron types at 5 weeks in vitro. Neurons from E12.5 CD-1 mice were combined with CPu neurons from E14.5 mice and the characteristics of striatal interneurons after 5 weeks in vitro were determined. All four major classes of interneurons were identified in these cultures as well as rare tyrosine hydroxylase positive interneurons. However, E14.5 mouse CPu cultures contained relatively few cholinergic interneurons rather than the nearly total absence seen in the rat. A later dissection day (E16.5) was required to obtain mouse CPu cultures totally lacking the cholinergic interneuron. We show that these cultures generated from two gestational age cells have much more nearly normal proportions of interneurons than the more common organotypic cultures of striatum. Interneurons are generated from both ages of embryos except for the cholinergic interneurons that originate from the medial ganglionic eminence of younger embryos. Study of these cultures should more accurately reflect neuronal processing as it occurs in the striatum in vivo. Furthermore, these results reveal a procedure for parallel culture of striatum and cholinergic depleted striatum that can be used to examine the function of the cholinergic interneuron in striatal networks. |
| Databáze: | OpenAIRE |
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