Aquaporin in different moult stages of a freshwater decapod crustacean: Expression and participation in muscle hydration control
| Autor: | Robert Tew Boyle, Carolina A. Freire, Monique Tomazele Rovani, Marta Marques Souza, Kamila Foguesatto |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Aquaporin Fresh Water Molting Aquaporins 010603 evolutionary biology 01 natural sciences Biochemistry 03 medical and health sciences Palaemonetes argentinus Decapoda Hemolymph Animals Molecular Biology biology Ecology Muscles Hydration control biology.organism_classification Crustacean Shrimp Cell biology 030104 developmental biology Gene Expression Regulation Moulting Tissue volume |
| Zdroj: | Comparative biochemistry and physiology. Part A, Molecularintegrative physiology. 208 |
| ISSN: | 1531-4332 |
| Popis: | Crustaceans, during their moult cycle, at the stages of both pre-moult and post-moult, need water uptake. This movement of water creates a challenge for the regulation of cell volume. The cells of freshwater decapods require a high regulatory capacity to deal with hyposmotic stresses, given the need to face dilution of the haemolymph during their moult cycles. This study investigated the variation in the expression of water channels (aquaporins) along the moult cycle of a freshwater palaemonid shrimp, focusing on their role in cell volume regulation. Moults in Palaemonetes argentinus have been investigated along three stages of its moult cycle: intermoult, late pre-moult and recent post-moult. For the evaluation of tissue volume regulation, the weight of isolatedmuscle, subjected to isosmotic and hyposmotic salines, was followed for 60min. The expression of AQP during the different moult stages was evaluated by immunocytochemistry. Muscle from the three moult stages in isosmotic conditions showed the same pattern of tissue volume regulation. When muscle from animals in pre-moult and intermoult were submitted to hyposmotic stress they swell, followed by volume regulation, while in post-moult the regulation is compromised. The difference in volume regulatory control between pre-moult and post-moult may be related to a possible regulation of water channels, as AQP expression was equal at these stages. This study presents novel findings for crustaceans in general, in the demonstration that AQP expression changes during the moult cycle of a decapod crustacean, together with the regulation of cell volume with the participation of AQPs. |
| Databáze: | OpenAIRE |
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