| Autor: |
Núria M. Pastor-Soler, Yidan Li, Brendan H. Grubbs, Zhongwei Li, Jingying Yu, Riana K. Parvez, Jill A. McMahon, Jyunhao Chen, Zipeng Zeng, Andrew P. McMahon, Albert D. Kim, Kenneth R. Hallows, Ariel Vonk, Tadrushi Patel, Elisabeth A. Rutledge, Matthew E. Thornton, Biao Huang |
| Rok vydání: |
2020 |
| Předmět: |
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| DOI: |
10.1101/2020.04.27.049031 |
| Popis: |
Kidney organoids model development and diseases of the nephron but not the contiguous epithelial network of the kidney’s collecting duct (CD) system. Here, we report the generation of an expandable, 3D branching ureteric bud (UB) organoid culture model that can be derived from primary UB progenitors from mouse and human fetal kidneys, or generatedde novofrom pluripotent human stem cells. UB organoids differentiate into CD organoidsin vitro, with differentiated cell types adopting spatial assemblies reflective of the adult kidney collecting system. Aggregating 3D-cultured nephron progenitor cells with UB organoidsin vitroresults in a reiterative process of branching morphogenesis and nephron induction, similar to kidney development. Combining efficient gene editing with the UB organoid model will facilitate an enhanced understanding of development, regeneration and diseases of the mammalian collecting system.One sentence summaryCollecting duct organoids derived from primary mouse and human ureteric bud progenitor cells and human pluripotent stem cells provide anin vitroplatform for genetic dissection of development, regeneration and diseases of the mammalian collecting system. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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