ALDH1 expression predicts progression of premalignant lesions to cancer in Type I endometrial carcinomas
| Autor: | Yahya Elshimali, Neda A. Moatamed, Madhuri Wadehra, Stephen P. Schettler, Vei Mah, Jamar P Uzzell, Alison Chu, Hania Shakeri, Jessica Tsui |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Kaplan-Meier Estimate
Stem cells Atypical hyperplasia 80 and over Atypia 2.1 Biological and endogenous factors Aetiology Cancer Aged 80 and over Tumor Multidisciplinary Tissue microarray Molecular medicine Middle Aged Prognosis Gene Expression Regulation Neoplastic Oncology Endometrial Hyperplasia Disease Progression Medicine Female Adult Science Aldehyde Dehydrogenase 1 Family Article Cell Line Uterine Cancer Clinical Research Cancer stem cell Cell Line Tumor Biomarkers Tumor medicine Humans Aged Neoplastic business.industry Endometrial cancer medicine.disease Endometrial Neoplasms Endometrial hyperplasia Gene Expression Regulation Cancer research business Precancerous Conditions Biomarkers |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) Scientific Reports Scientific reports, vol 11, iss 1 |
| ISSN: | 2045-2322 |
| Popis: | In type 1 endometrial cancer, unopposed estrogen stimulation is thought to lead to endometrial hyperplasia which precedes malignant progression. Recent data from our group and others suggest that ALDH activity mediates stemness in endometrial cancer, but while aldehyde dehydrogenase 1 (ALDH1) has been suggested as a putative cancer stem cell marker in several cancer types, its clinical and prognostic value in endometrial cancer remains debated. The aim of this study was to investigate the clinical value of ALDH1 expression in endometrial hyperplasia and to determine its ability to predict progression to endometrial cancer. Interrogation of the TCGA database revealed upregulation of several isoforms in endometrial cancer, of which the ALDH1 isoforms collectively constituted the largest group. To translate its expression, a tissue microarray was previously constructed which contained a wide sampling of benign and malignant endometrial samples. The array contained a metachronous cohort of samples from individuals who either developed or did not develop endometrial cancer. Immunohistochemical staining was used to determine the intensity and frequency of ALDH1 expression. While benign proliferative and secretory endometrium showed very low levels of ALDH1, slightly higher expression was observed within the stratum basalis. In disease progression, cytoplasmic ALDH1 expression showed a step-wise increase between endometrial hyperplasia, atypical hyperplasia, and endometrial cancer. ALDH1 was also shown to be an early predictor of EC development, suggesting that it can serve as an independent prognostic indicator of patients with endometrial hyperplasia with or without atypia who would progress to cancer (p = 0.012). |
| Databáze: | OpenAIRE |
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