Oxytocin and vasopressin gene expression and RNA splicing patterns in the rat supraoptic nucleus
| Autor: | Raymond L. Fields, Chunmei Yue, Harold Gainer, Todd A. Ponzio |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Vasopressin Vasopressins Physiology RNA Splicing In situ hybridization Biology Oxytocin Supraoptic nucleus Rats Sprague-Dawley Gene expression Genetics medicine Animals Gene In Situ Hybridization Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Molecular biology Rats Cell biology Hypothalamus RNA splicing Female Supraoptic Nucleus Research Article medicine.drug |
| Zdroj: | Physiological Genomics. 35:231-242 |
| ISSN: | 1531-2267 1094-8341 |
| Popis: | In this study, we test the hypothesis that there are differential splicing patterns between the expressed oxytocin (OT) and vasopressin (VP) genes in the rat supraoptic nucleus (SON). We quantify the low abundance, intron-containing heteronuclear RNAs (hnRNAs) and the higher abundance mRNAs in the SON using two-step, quantitative SYBR Green real-time reverse transcription (RT)-PCR and external standard curves constructed using synthetic 90 nt sense-strand oligonucleotides. The levels of OT and VP mRNA in the SON were found to be similar, ∼108 copies/SON pair, whereas the copy numbers of VP hnRNAs containing intron 1 or 2 and the OT hnRNA containing intron 1 are much lower, i.e., ∼102–103 copies/rat SON pair. However, the estimated copy number of the intron 2-containing OT hnRNA is much larger, ∼106 copies/SON pair. The relative distributions of all the OT and VP RNA species were invariant and independent of the physiological status of the rats (e.g., osmotically stimulated or lactating rats). Using intron-specific riboprobes against hnRNAs, we demonstrate by fluorescence in situ hybridization strong signals of OT hnRNA containing intron 2 predominantly in the cytoplasm, in contrast to the localization of the VP hnRNA found only in the nuclei. Taken together, these data support the view that the splicing patterns between OT and VP gene transcripts are different and show that there is a selective cytoplasmic retention of OT intron 2. |
| Databáze: | OpenAIRE |
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