Induction of ovarian primordial follicle assembly by connective tissue growth factor CTGF
| Autor: | Eric E. Nilsson, Michael K. Skinner, Ryan Schindler |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
medicine.medical_specialty
Developmental Biology/Germ Cells Connective tissue lcsh:Medicine Ovary Apoptosis Biology Organ culture Andrology Rats Sprague-Dawley Transforming Growth Factor beta1 Follicle Developmental Biology/Molecular Development Ovarian Follicle Internal medicine medicine Developmental Biology/Developmental Molecular Mechanisms Animals Ovarian follicle lcsh:Science Developmental Biology/Organogenesis Multidisciplinary integumentary system Developmental Biology/Morphogenesis and Cell Biology lcsh:R Connective Tissue Growth Factor Oocyte Rats CTGF Endocrinology medicine.anatomical_structure Gene Expression Regulation Oocytes Developmental Biology/Cell Differentiation lcsh:Q Female Folliculogenesis Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 5, Iss 9, p e12979 (2010) |
| ISSN: | 1932-6203 |
| Popis: | Primordial follicle assembly is a process that occurs when oocyte nests break down to form individual primordial follicles. The size of this initial pool of primordial follicles in part determines the reproductive lifespan of the female. Connective tissue growth factor (CTGF) was identified as a potential regulatory candidate for this process in a previous microarray analysis of follicle development. The current study examines the effects of CTGF and associated transforming growth factor beta 1 (TGFβ-1) on follicle assembly. Ovaries were removed from newborn rat pups and placed in an organ culture system. The ovaries treated with CTGF for two days were found to have an increased proportion of assembled follicles. CTGF was found to regulate the ovarian transcriptome during primordial follicle assembly and an integrative network of genes was identified. TGFβ-1 had no effect on primordial follicle assembly and in combination with CTGF decreased oocyte number in the ovary after two days of culture. Over ten days of treatment only the combined treatment of CTGF and TGFβ-1 was found to cause an increase in the proportion of assembled follicles. Interestingly, treatment with TGFβ-1 alone resulted in fewer total oocytes in the ovary and decreased the primordial follicle pool size after ten days of culture. Observations indicate that CTGF alone or in combination with TGFβ-1 stimulates primordial follicle assembly and TGFβ-1 can decrease the primordial follicle pool size. These observations suggest the possibility of manipulating primordial follicle pool size and influencing female reproductive lifespan. |
| Databáze: | OpenAIRE |
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