Investigating electron-transfer processes using a biomimetic hybrid bilayer membrane system
| Autor: | Hong-Yuan Chen, Yi-Lun Ying, Hao Zhou, Yi-Tao Long, Todd C. Sutherland, Wei Ma, Zhen Gu, Da-Wei Li, Li-Xia Qin |
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| Rok vydání: | 2013 |
| Předmět: |
Lipid Bilayers
Microscopy Atomic Force Spectrum Analysis Raman Redox General Biochemistry Genetics and Molecular Biology Electron Transport Electron transfer Biomimetics Electrochemistry Electrodes chemistry.chemical_classification Chemistry Biomolecule Bilayer Photoelectron Spectroscopy Biological membrane Membranes Artificial NAD Electron transport chain Dielectric spectroscopy Membrane Chemical engineering Dielectric Spectroscopy Biophysics Gold Oxidation-Reduction |
| Zdroj: | Nature protocols. 8(3) |
| ISSN: | 1750-2799 |
| Popis: | Here we report a protocol to investigate the electron-transfer processes of redox-active biomolecules in biological membranes by electrochemistry using biomimetic hybrid bilayer membranes (HBMs) assembled on gold electrodes. Redox-active head groups, such as the ubiquinone moiety, are embedded in HBMs that contain target molecules, e.g., nicotinamide adenine dinucleotide (NADH). By using this approach, the electron-transfer processes between redox molecules and target biomolecules are mediated by mimicking the redox cycling processes in a natural membrane. Also included is a procedure for in situ surface-enhanced Raman scattering (SERS) to confirm the electrochemically induced conformational changes of the target biomolecules in the HBMs. In addition, each step in constructing the HBMs is characterized by electrochemical impedance spectroscopy (EIS), high-resolution X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). The time required for the entire protocol is ∼12 h, whereas the electrochemical measurement of electron-transfer processes takes less than 1 h to complete. |
| Databáze: | OpenAIRE |
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