The expression of IL10RA in colorectal cancer and its correlation with the proliferation index and the clinical stage of the disease
| Autor: | Magdalena Czuba, Michał Kulus, Tomasz Jurek, Natalia Glatzel-Plucinska, Aleksandra Piotrowska, Krzysztof Kurnol, Dariusz Janczak, Łukasz Zadka, Piotr Dziegiel, Aleksandra Nowak, Mariusz Chabowski |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male STAT3 Transcription Factor 0301 basic medicine Oncology medicine.medical_specialty Proliferation index Colorectal cancer Immunology medicine.disease_cause Biochemistry Interleukin 10 receptor alpha subunit Pathogenesis 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Humans Immunology and Allergy Receptors Interleukin-10 Stage (cooking) Molecular Biology Aged Cell Proliferation Aged 80 and over Tissue microarray business.industry Hematology Middle Aged medicine.disease Interleukin-10 Ki-67 Antigen 030104 developmental biology 030220 oncology & carcinogenesis Immunohistochemistry Female Colorectal Neoplasms Carcinogenesis business |
| Zdroj: | Cytokine. 110:116-125 |
| ISSN: | 1043-4666 |
| DOI: | 10.1016/j.cyto.2018.04.030 |
| Popis: | Despite the widely described role of IL10 in immune response regulation during carcinogenesis, there is no established model describing the role of its receptor. The aim of this study is to elucidate the relationship between the subunit alpha of IL10 receptor (IL10RA) in the pathogenesis of colorectal cancer (CRC).The study was conducted on archived paraffin blocks of 125 CRC patients, from which tissue microarrays (TMA) were made. These were subsequently used for immunohistochemistry to assess the expression of IL10RA, IL10, phosphorylated STAT3 (pSTAT3) and the Ki67 proliferation index. The intensity of both reactions was assessed by independent researchers using two approaches: digital image analysis and the Remmele and Stegner score (IRS). To assess the possible correlations between the two investigated markers and the clinical stage of CRC, the Pearson correlation coefficient was calculated. The expression of aforementioned proteins was assessed in tumor samples, healthy surgical margins and healthy control samples, obtained from cadavers during autopsy from the Department of Forensic Medicine. Statistical analysis was conducted using Statistica ver. 13.05 software.The final analysis included 105 CRC patients with complete clinical and pathological data, for whom the expressions of IL10RA, IL10, pSTAT3 and Ki67 were assessed using two independent methods. There was a positive correlation between the IL10RA expression and Ki-67 proliferation index (R = 0.63, p 0.001) and a negative correlation between the IL10RA expression and the clinical stage of CRC (R = -0.21, p = 0.022). IL10RA correlated positively with pSTAT3 and IL10 in neoplastic tissue and tumor margin (with p 0.01 for all correlations). We also observed a significantly higher expression of IL10RA in healthy surgical margins when compared to the actual tumor (p = 0.023, the paired t-test). The expression of IL10 was significantly higher in tumors than in healthy intestinal endothelium from control group.The correlations between the expression of IL10RA and the proliferation index or the clinical stage of CRC seem to confirm the importance of IL10RA in the pathogenesis of CRC. The higher expression of IL10RA in healthy surgical margins than in the tumor itself may suggest that IL10RA plays a role in regulating immune response to the neoplasm. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect