Alginate-based 3D cancer cell culture for therapeutic response modeling
| Autor: | Samar Ghorbanpoor, Xiao Pan, Giovanna Stein Crowther, Henning Willers, Ellen Murchie, Farideh Davoudi, Satoshi Yoda, Xunqin Yin, Cyril H. Benes, Aaron N. Hata |
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| Rok vydání: | 2021 |
| Předmět: |
Science (General)
Alginates Cell Survival High-throughput screening Tumor cells Antineoplastic Agents Biology Models Biological General Biochemistry Genetics and Molecular Biology Q1-390 Calcium Chloride In vivo Cell Line Tumor medicine Protocol Humans Cell Culture Techniques Three Dimensional Cellular organization Cell Proliferation Cancer General Immunology and Microbiology General Neuroscience Cell-based assays Cell based assays medicine.disease Cell culture Cancer cell Cancer research |
| Zdroj: | STAR Protocols STAR Protocols, Vol 2, Iss 2, Pp 100391-(2021) |
| ISSN: | 2666-1667 |
| Popis: | Summary Two-dimensional (2D) culture of tumor cells fails to recapitulate some important aspects of cellular organization seen in in vivo experiments. In addition, cell cultures traditionally use non-physiological concentration of nutrients. Here, we describe a protocol for a facile three-dimensional (3D) culture format for cancer cells. This 3D platform helps overcome the 2D culture limitations. In addition, it allows for longitudinal modeling of responses to cancer therapeutics. For complete details on the use and execution of this protocol, please refer to Lhuissier et al. (2017), Lehmann et al. (2016), Liu et al. (2016), and Duval et al. (2011). Graphical Abstract Highlights • A detailed protocol on hydrogel-based 3D culture of patient-derived tumor cell lines • No binding sites for cells in hydrogel polymers allowing for pure interaction of cells • Longitudinal 3D proliferation assays and drug-response assessments • Quick and easy recovery of 3D-cultured cells for downstream experiments Two-dimensional (2D) culture of tumor cells fails to recapitulate some important aspects of cellular organization seen in in vivo experiments. In addition, cell cultures traditionally use non-physiological concentration of nutrients. Here, we describe a protocol for a facile three-dimensional (3D) culture format for cancer cells. This 3D platform helps overcome the 2D culture limitations. In addition, it allows for longitudinal modeling of responses to cancer therapeutics. |
| Databáze: | OpenAIRE |
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