Population Genetic and Functional Analysis of a cis-Regulatory Polymorphism in the Drosophila melanogaster Metallothionein A gene
| Autor: | Timothy J S Ramnarine, Ana Catalán, Amanda Glaser-Schmitt, John Parsch |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Untranslated region Male lcsh:QH426-470 Population cis-regulation Population genetics Regulatory Sequences Nucleic Acid 010603 evolutionary biology 01 natural sciences Article untranslated region 03 medical and health sciences INDEL Mutation Polymorphism (computer science) Stress Physiological Gene expression Genetics Animals oxidative stress deletion Genetik Indel education Gene Drosophila melanogaster Genetics (clinical) Africa South of the Sahara Alleles Sequence Deletion education.field_of_study Polymorphism Genetic biology population genetics biology.organism_classification Adaptation Physiological lcsh:Genetics 030104 developmental biology Genetics Population Gene Expression Regulation indel gene expression Female Metallothionein |
| Zdroj: | Genes Volume 10 Issue 2 Genes, Vol 10, Iss 2, p 147 (2019) |
| Popis: | Although gene expression can vary extensively within and among populations, the genetic basis of this variation and the evolutionary forces that maintain it are largely unknown. In Drosophila melanogaster, a 49-bp insertion/deletion (indel) polymorphism in the Metallothionein A (MtnA) gene is associated with variation in MtnA expression and oxidative stress tolerance. To better understand the functional and evolutionary significance of this polymorphism, we investigated it in several worldwide populations. In a German population, the deletion was present at a high and stable frequency over multiple seasons and years, and was associated with increased MtnA expression. There was, however, no evidence that the polymorphism was maintained by overdominant, seasonally fluctuating, or sexually antagonistic selection. The deletion was rare in a population from the species&rsquo ancestral range in sub-Saharan Africa and is likely the result of non-African admixture, suggesting that it spread to high frequency following the species&rsquo out-of-Africa expansion. Using data from a North American population, we found that the deletion was associated with MtnA expression and tolerance to oxidative stress induced by menadione sodium bisulfite. Our results are consistent with the deletion being selectively favored in temperate populations due to the increased MtnA expression and oxidative stress tolerance that it confers. |
| Databáze: | OpenAIRE |
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