Escherichia coli mutants deficient in exoribonucleases
| Autor: | Nikolai Nikolaev, Virginia Folsom, David Schlessinger |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Exonuclease
Messenger RNA Strain (chemistry) Mutant Biophysics Mutagenesis (molecular biology technique) Cell Biology Ribosomal RNA Biology Biochemistry Molecular biology Microbiology Exoribonucleases Kinetics Ribonucleases Mutation biology.protein Escherichia coli Doubling time Molecular Biology Nitrosoguanidines |
| Zdroj: | Biochemical and biophysical research communications. 70(3) |
| ISSN: | 0006-291X |
| Popis: | Strain S296, isolated by screening 2000 colonies after nitrosoguanidine mutagenesis, yields extracts with less than 1% of wild-type RNase activity against ( 3 H) poly(U). Unlike other E. coli strains, S296 grows with a doubling time of about 2 hr., both in nutrient broth and in minimal medium, and at 30°, 37° and 42°. The strain retains 10 to 20% of wild-type exonuclease activity against ( 3 H) rRNA or T4 phage-specific mRNA; but two further mutants, made by screening mutagenized colonies of strain S296, are reduced to 3% of wild-type activity against those substrates as well. |
| Databáze: | OpenAIRE |
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