A Dual, Systematic Approach to Malaria Diagnostic Biomarker Discovery
| Autor: | Xavier C. Ding, Ewurama D. A. Owusu, Robert Kirk DeLisle, Seda Yerlikaya, Augustina Frimpong |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Plasmodium falciparum malaria Protozoan Proteins Antigens Protozoan Computational biology Proteomics Sensitivity and Specificity World health Uncomplicated malaria parasitic diseases Major Article diagnostics Global health medicine Humans Diagnostic biomarker Malaria Falciparum Rapid diagnostic test GAPDH DHFR-TS Diagnostic Tests Routine business.industry medicine.disease Biomarker (cell) AcademicSubjects/MED00290 Infectious Diseases biomarker business Biomarkers Malaria |
| Zdroj: | Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America |
| ISSN: | 1537-6591 1058-4838 |
| Popis: | Background The emergence and spread of Plasmodium falciparum parasites that lack HRP2/3 proteins and the resulting decreased utility of HRP2-based malaria rapid diagnostic tests (RDTs) prompted the World Health Organization and other global health stakeholders to prioritize the discovery of novel diagnostic biomarkers for malaria. Methods To address this pressing need, we adopted a dual, systematic approach by conducting a systematic review of the literature for publications on diagnostic biomarkers for uncomplicated malaria and a systematic in silico analysis of P. falciparum proteomics data for Plasmodium proteins with favorable diagnostic features. Results Our complementary analyses led us to 2 novel malaria diagnostic biomarkers compatible for use in an RDT format: glyceraldehyde 3-phosphate dehydrogenase and dihydrofolate reductase-thymidylate synthase. Conclusions Overall, our results pave the way for the development of next-generation malaria RDTs based on new antigens by identifying 2 lead candidates with favorable diagnostic features and partially de-risked product development prospects. The World Health Organization called for the identification of novel biomarkers that can fill the diagnostic gap in settings where hrp2/3 deletions are common. By adopting a dual approach, we identified 2 candidates, glyceraldehyde 3-phosphate dehydrogenase and dihydrofolate reductase-thymidylate synthase, with favorable diagnostic features. |
| Databáze: | OpenAIRE |
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