Correlation of beta-catenin and cyclin D1 expression in colon cancers
| Autor: | Mitsugu Sekimoto, Yoshiyuki Fujiwara, Takehiro Utsunomiya, Morito Monden, Hiroyoshi Takemoto, Hitoshi Shiozaki, Yuichiro Doki, Shigeyuki Tamura, Masahiko Yano, Takushi Yasuda |
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| Rok vydání: | 2001 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty animal structures Beta-catenin Cyclin D Blotting Western Adenocarcinoma Cyclin D1 Transcription (biology) medicine Biomarkers Tumor Humans beta Catenin Retrospective Studies Oncogene biology Cadherin business.industry Cell adhesion molecule Cell Cycle General Medicine Cadherins Neoplasm Proteins Cytoskeletal Proteins Cell Transformation Neoplastic Oncology Catenin embryonic structures Colonic Neoplasms biology.protein Cancer research Trans-Activators business |
| Zdroj: | Oncology. 61(3) |
| ISSN: | 0030-2414 |
| Popis: | Objective: Beta-catenin activates transcription by TCF/LEF and has been regarded as an oncogene in a wide range of malignant tumors. Among various molecules regulated by beta-catenin/Tcf, cyclin D1 is the most likely candidate for stimulation of the oncogenic pathway. The association between beta-catenin and cyclin D1 was investigated using clinical samples from colorectal cancers. Methods: The expression of beta-catenin and cyclin D1 was investigated by immunohistochemical analyses of samples from 70 patients with colorectal cancers. In 28 of the fresh tumor samples, beta-catenin protein was separated into soluble and insoluble fractions and quantitatively correlated with cyclin D1 protein by Western blot analysis. Results: Compared with noncancerous epithelium, beta-catenin and cyclin D1 were overexpressed (+) in 35 (50%) and 30 cases (43%), respectively. Cyclin D1 (+) was observed in 74% (26/35) of beta-catenin (+) cases, but only in 11% (4/35) of the beta-catenin (–) cases. Thus, there was a strong association between the expression of beta-catenin and that of cyclin D1 (p < 0.001). In the Western blot analysis, the amount of cyclin D1 correlated well with beta-catenin expression in the soluble fraction (p = 0.0016), but not with beta-catenin in the insoluble fraction or with E-cadherin expression. Beta-catenin (–)/cyclin D1 (–) cases displayed less tumor invasion than the remaining cases. However, there were no significant differences in lymph node metastasis or other clinicopathological findings. Conclusion: Our results indicate that beta-catenin overexpression in the cytoplasm may promote malignant transformation by triggering cyclin D1 expression in colorectal cancers. |
| Databáze: | OpenAIRE |
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