Some properties of the Epithelioma papulosum cyprini (EPC) cell line from carp cyprinus carpio
| Autor: | D. Muzinić, J.F. Vautherot, L. O. Zwillenberg, D. Sulimanovic, M. Bearzotti, S. Chilmonczyk, N. Fijan, P. de Kinkelin |
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| Rok vydání: | 1983 |
| Předmět: |
Virus herpès
Pathologie des poissons Infectious hematopoietic necrosis virus Karyotype Article Rhabdoviruses Virus Cyprinus Epithelioma papulosum cyprini Cyprinidae medicine Herpes virus Caryotype Carp Culture cellulaire Carp pox Novirhabdovirus biology Epithelioma Rhabdovirus General Medicine biology.organism_classification medicine.disease Virology Variole de la carpe Vesicular stomatitis virus Fish pathology Cell culture |
| Zdroj: | Annales De L'Institut Pasteur. Virology |
| ISSN: | 0769-2617 |
| DOI: | 10.1016/s0769-2617(83)80060-4 |
| Popis: | Summary A cell line, named Epithelioma papulosum cyprini (EPC) and originating from carp epidermal herpes virus-induced hyperplastic lesions, was esta blished and has now given rise to more than 80 subcultures. It grows within a wide temperature range (15–33°C), survives between 7 and 10°C for several months without alterations in its further virus susceptibility and growth characteristics, and degenerates at 37°C. Its karyotype is 2n=96. It propagates most of the systemic infection viruses of the fish families Salmonidae, Cyprinidae, Anguillidae and Esocidae, as well as Indiana-type vesicular stomatitis virus. Despite the origin of EPC cells, we were unable to demonstrate the presence of herpes virus in them. Their transformation was reflected in their karyotype (normally 2n=100) and by their growth ability in soft agar medium; monolayer growth, however, was inhibited at a density of 3×106 cells/cm2. The temperature growth range, good splitting ratio (1/10) and virus susceptibility make EPC cells a highly suitable material both for fish pathology and for comparative virology studies. |
| Databáze: | OpenAIRE |
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