Effects of 5-(3-methyl-1-triazeno)imidazole-4-carboxamide (NSC-407347), an alkylating agent derived from 5-(3,3-dimethyl-1-triazeno)imidazole-4-carboxamide (NSC-45388)
| Autor: | Richard W. Decker, Nobuko S. Mizuno, Baiba Zakis |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Alkylating Agents
Time Factors DNA polymerase medicine.drug_class Guanine Carboxamide Antineoplastic Agents Biochemistry chemistry.chemical_compound Mice L Cells RNA polymerase medicine Animals Cells Cultured Pharmacology biology Cell growth RNA DNA Templates Genetic Fibroblasts Molecular biology In vitro Dacarbazine chemistry biology.protein Cattle Triazenes Cell Division |
| Zdroj: | Biochemical pharmacology. 24(5) |
| ISSN: | 0006-2952 |
| Popis: | All experiments were performed in the absence of light. A 5-(3-methyl-1-triazeno)imidazole-4-carboxamide (MIC) concentration of less than 10 −4 M had no effect on cell growth of L cells. At higher concentrations, the cells were inhibited to levels which were similar to those obtained with equimolar doses of 5-(3,3-dimethyl-1-triazeno)imidazole-4-carboxamide (DIC). MIC inhibited the incorporation of 3 H-thymidine by DNA more than that of 3 H-uridine by RNA. Uptake of 3 H from 3 H-methyl-MIC by DNA was not influenced by the stage of the cell cycle. The greatest binding took place with DNA of the euchromatin fraction. MIC-treated DNA exhibited impaired template activity in vitro in the RNA polymerase s ystem but not with that of DNA polymerase. Chromatography of DNA hydrolysate from 3 H-methyl-MIC-treated cells showed three major radioactive peaks, which corresponded to adenine, guanine and 7-methylguanine. Hydroxyurea markedly reduced the uptake of 3 H by adenine and guanine but had relatively little effect on the 3 H content of 7-methylguanine. Similarity of cytotoxic reactions of MIC to those of DIC supports the thesis that in the animal system DIC is metabolically converted to MIC, a potential methylating agent. Many of the effects of DIC can be accounted for by the action of MIC. |
| Databáze: | OpenAIRE |
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