Expansión y cambios fenotípicos de células mesenquimales de médula ósea de ratón cultivadas con FGF-2 y medio condicionado por el nervio facial
| Autor: | Hécio Henrique Araújo de-Morais, Miriam Stela Maris de Oliveira Costa, Eduardo Pereira de Azevedo, Dayane Pessoa de Araújo, Marco Antonio Botelho, Fausto Pierdoná Guzen, Carlos Augusto Galvão Barboza, Amália Cínthia Meneses Rêgo, Irami Araújo Filho, Dinalva Brito de Queiroz, José Rodolfo Lopes de Paiva Cavalcanti, Eudes Euler de Souza Lucena, Jeferson Sousa Cavalcante, Expedito Silva do Nascimento Júnior |
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| Rok vydání: | 2018 |
| Předmět: |
Plasticity
Fibroblast growth factor 2 Mesenchymal stem cell Immunocytochemistry Transdifferentiation Trans-differentiation Facial nerve injury Biology Fibroblast growth factor Facial nerve Cell biology Paracrine signalling medicine.anatomical_structure nervous system biology.protein medicine Mesenchymal stem cells Bone marrow Anatomy NeuN Conditioned medium |
| Zdroj: | Repositório Institucional da UFRN Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN International Journal of Morphology v.36 n.3 2018 SciELO Chile CONICYT Chile instacron:CONICYT |
| ISSN: | 0717-9502 |
| Popis: | SUMMARY: Mesenchymal cells (MCs) exhibit great regenerative potential due to their intrinsic properties and ability to restore tissue function, either directly through transdifferentiation or indirectly through paracrine effects. This study aimed to evaluate morphometric and phenotypic changes in MCs grown with facial nerve-conditioned medium in the presence or absence of fibroblast growth factor 2 (FGF-2). For quantitative phenotypic analysis, the expression of GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200 was analyzed by immunocytochemistry. Cells cultured with facial nerve-conditioned medium in the presence of FGF-2 expressed GFAP, OX-42, MAP-2, β-tubulin III, NeuN, and NF-200. On average, the area and perimeter of GFAP-positive cells were higher in the group cultured with facial nerve-conditioned medium compared to the group cultured with conditioned medium and FGF-2 (p=0.0001). This study demonstrated the plasticity of MCs for neuronal and glial lineages and opens up new research perspectives in cell therapy and trans.differentiation. |
| Databáze: | OpenAIRE |
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