Platelet secretory phospholipase A2 fails to induce rabbit platelet activation and to release arachidonic acid in contrast with venom phospholipases A2
| Autor: | Cassian Bon, Hubertus M. Verheij, Lhousseine Touqui, Peet A. Franken, B. Boris Vargaftig, Carine Mounier |
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| Rok vydání: | 1994 |
| Předmět: |
Blood Platelets
Male Platelet Aggregation Swine Biophysics Phospholipid Phospholipase Pharmacology Biochemistry Phospholipases A Substrate Specificity chemistry.chemical_compound Endocrinology Phospholipase A2 Phosphatidylcholine Animals Platelet Platelet activation Pancreas Phospholipase A Arachidonic Acid biology Platelet Activation Phospholipases A2 chemistry biology.protein Cattle lipids (amino acids peptides and proteins) Arachidonic acid Rabbits Snake Venoms |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 1214:88-96 |
| ISSN: | 0005-2760 |
| Popis: | The ability of platelet secretory phospholipase A2 (sPLA2) to induce platelet activation was investigated. sPLA2 (group II) contained in an activated platelet supernatant, as well as high concentrations of purified recombinant platelet sPLA2, failed to induce platelet activation. Furthermore, sPLA2 did not modify platelet activation induced by various agonists. The possible relationship between the failure of this enzyme to induce platelet activation and its origin (mammalian) or its structural group (group II) was then investigated, using pancreatic PLA2s (group I) and venom PLA2s from groups I, II and III. All venom PLA2s induced platelet activation that was accompanied by the liberation of arachidonic acid and was abolished by aspirin. In contrast, as observed for platelet sPLA2, enzymes from hog or bovine pancreas were unable to induce platelet activation even when used at high concentrations. Interestingly, PLA2 able to induce platelet activation efficiently hydrolyse phosphatidylcholine, while those inactive on platelets did not. Taken together, these results suggest that the catalytic activity of added PLA2 is necessary but not sufficient to induce platelet activation. Moreover, the ability of PLA2 to induce platelet activation is not related to its structural group (I, II, III) but rather to its origin (venom vs. mammalian) and capacity to hydrolyse phosphatidylcholine, the major phospholipid of the outer leaflet of the plasma membrane. |
| Databáze: | OpenAIRE |
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