Isolation, genomic organization, and expression analysis of Men1, the murine homolog of the MEN1 gene
| Autor: | Francis S. Collins, Nijaguna B. Prasad, Settara C. Chandrasekharappa, Judy S. Crabtree, Kevin D. Brown, William J. Pavan, Allen M. Spiegel, Danny Wangsa, A. Lee Burns, Pachiappan Manickam, Stephen J. Marx, Siradanahalli C. Guru, Karen J. Dunn |
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| Rok vydání: | 1999 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities endocrine system DNA Complementary endocrine system diseases Molecular Sequence Data Locus (genetics) Biology Exon Mice Proto-Oncogene Proteins Genetics medicine Animals Humans MEN1 Amino Acid Sequence Cloning Molecular Deoxyribonucleases Type II Site-Specific Gene In Situ Hybridization In Situ Hybridization Fluorescence Genomic organization medicine.diagnostic_test Sequence Homology Amino Acid Cell Cycle Intron Chromosome Mapping Gene Expression Regulation Developmental Exons Sequence Analysis DNA Blotting Northern Embryo Mammalian Molecular biology Introns Neoplasm Proteins Open reading frame Fluorescence in situ hybridization |
| Zdroj: | Mammalian genome : official journal of the International Mammalian Genome Society. 10(6) |
| ISSN: | 0938-8990 |
| Popis: | The mouse homolog of the human MEN1 gene, which is defective in a dominant familial cancer syndrome, multiple endocrine neoplasia type 1 (MEN1), has been identified and characterized. The mouse Men1 transcript contains an open reading frame encoding a protein of 611 amino acids which has 97% identity and 98% similarity to human menin. Sequence of the entire Men1 gene (9.3 kb) was assembled, revealing 10 exons, with exon 1 being non-coding; a polymorphic tetranucleotide repeat was located in the 5'- flanking region. The exon-intron organization and the size of the coding exons 2-9 were well conserved between the human and mouse genes. Fluorescence in situ hybridization localized the Men1 gene to mouse Chromosome (Chr) 19, a region known to be syntenic to human Chr 11q13, the locus for the MEN1 gene. Northern analysis indicated two messages-2.7 kb and 3.1 kb-expressed in all stages of the embryo analyzed and in all eight adult tissues tested. The larger transcript differs from the smaller by the inclusion of an unspliced intron 1. Whole-mount in situ hybridization of 10.5-day and 11.5-day embryos showed ubiquitous expression of Men1 RNA. Western analysis with antibodies raised against a conserved C-terminal peptide identified an approximately 67-kDa protein in the lysates of adult mouse brain, kidney, liver, pancreas, and spleen tissues, consistent with the size of human menin. The levels of mouse menin do not appear to fluctuate during the cell cycle. |
| Databáze: | OpenAIRE |
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