A Mathematical Model for Assessing Changes in Neurofilament Protein Levels in Neurites and Cell Bodies of Differentiating Neuroblastoma Cells
| Autor: | Lynda Fletcher, Elizabeth H McFarlane, Keith Fletcher, Iain C. Campbell, Paul A.A. Grant |
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| Rok vydání: | 2000 |
| Předmět: |
Statistics and Probability
Neurofilament Neurite Cellular differentiation Models Neurological Cell Population Enzyme-Linked Immunosorbent Assay Biology General Biochemistry Genetics and Molecular Biology Neuroblastoma Differentiating Neuroblastoma Neurofilament Proteins Neurites Tumor Cells Cultured medicine Humans Cytoskeleton education education.field_of_study General Immunology and Microbiology Applied Mathematics Cell Differentiation General Medicine medicine.disease Molecular biology Cell biology medicine.anatomical_structure Modeling and Simulation General Agricultural and Biological Sciences |
| Zdroj: | Journal of Theoretical Biology. 205:241-252 |
| ISSN: | 0022-5193 |
| Popis: | A mathematical model which allows the calculation of the level of neurofilament protein in the cell body (x) and in the neurites (y) of differentiating SK-N-SH cells is presented. The model considers the changes in cell number (proliferating cells) and the number of cells with neurites (differentiating cells). It takes into account the fact that (i) when cells are cultured in differentiating conditions, an increase in cell number is initially observed and (ii) in a non-synchronized population of differentiating cells, the length of neurite extended by individual cells varies within the population. Total neurofilament protein levels in a population of cells were measured by enzyme-linked immunoabsorbant assay and application of the model to the data allowed values for x and y to be calculated. The validity of the model is supported by the fact that the predicted total neurofilament protein levels are highly correlated with the experimentally derived neurofilament protein levels. The model should be of use in temporal studies of cytoskeletal proteins involved in neuronal growth/differentiation and also in studies in which the system is a target of toxic insult. |
| Databáze: | OpenAIRE |
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