TNF receptor gene therapy results in suppression of IgG2a anticollagen antibody in collagen induced arthritis
| Autor: | Bin Wu, Paul D. Robbins, Paul H. Wooley, P. Mukherjee, Seon Hee Kim, Lois Mayton |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
musculoskeletal diseases
Genetic enhancement Genetic Vectors Immunology Arthritis Inflammation Receptors Tumor Necrosis Factor General Biochemistry Genetics and Molecular Biology Arthritis Rheumatoid Mice Rheumatology medicine Animals Immunology and Allergy Collagen Type II Autoantibodies Immunosuppression Therapy Autoimmune disease Tumor Necrosis Factor-alpha business.industry Autoantibody Genetic Therapy Th1 Cells medicine.disease Arthritis Experimental Connective tissue disease Extended Report Retroviridae Mice Inbred DBA Immunoglobulin G Rheumatoid arthritis Female Tumor necrosis factor alpha medicine.symptom business |
| Zdroj: | Annals of the Rheumatic Diseases. 62:707-714 |
| ISSN: | 0003-4967 |
| DOI: | 10.1136/ard.62.8.707 |
| Popis: | Background: Therapeutic strategies to block tumour necrosis factor α (TNFα) activity in experimental autoimmune arthritis models and rheumatoid arthritis (RA) have proved highly successful, and provide sustained beneficial effects. Objective: To examine whether TNFα inhibition has immunological activity beyond the reduction of inflammation in collagen induced arthritis (CIA), an established experimental model of RA. Methods: Arthritic DBA/1 mice received single periarticular injections of retroviral constructs encoding human TNF receptor (TNF-R) into the affected arthritic paw, at the onset of arthritis. Severity of arthritis, antibodies to collagen type II (CII), and extent of pathological joint damage of arthritic paws were compared between TNF-R and media treated (control) animals 3, 7, 14, 21, and 49 days after disease onset. Results: Severity of CIA was significantly decreased in TNF-R treated animals compared with controls, 14–34 days after disease onset. Joint destruction was reduced in TNF-R injected joints and in the uninjected contralateral and ipsilateral paws of TNF-R treated animals. Seven days after disease onset, TNF-R treated mice had lower levels of inflammatory Th1 driven IgG2a antibodies to CII (p |
| Databáze: | OpenAIRE |
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