OxLDL-IgG immune complexes induce expression and secretion of proatherogenic cytokines by cultured human mast cells
| Autor: | Petri T. Kovanen, Riina Oksjoki, Jani Lappalainen, Ken Lindstedt |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Time Factors
medicine.medical_treatment Inflammation Tryptase Antigen-Antibody Complex Biology Histamine Release chemistry.chemical_compound medicine Humans Mast Cells RNA Messenger Cells Cultured Chemokine CCL2 Tumor Necrosis Factor-alpha Monocyte Interleukin-8 Atherosclerosis Mast cell Lipoproteins LDL Interleukin 33 medicine.anatomical_structure Cytokine Gene Expression Regulation chemistry Immunoglobulin G Immunology biology.protein Cytokines Tryptases lipids (amino acids peptides and proteins) Tumor necrosis factor alpha Inflammation Mediators medicine.symptom Cardiology and Cardiovascular Medicine Histamine Signal Transduction |
| Zdroj: | Atherosclerosis. 214:357-363 |
| ISSN: | 0021-9150 |
| Popis: | Objective Human atherosclerotic lesions contain mast cells and immunoglobulin G immune complexes containing oxidized low-density lipoproteins (oxLDL-IgG ICs). Here we studied whether such oxLDL-IgG ICs can activate human mast cells and induce them to express and secrete pro-inflammatory cytokines that are potentially capable of inducing and amplifying atherogenic processes. Methods and results Incubation of cultured human mast cells in the presence of oxLDL-IgG ICs led to a significant dose-dependent upregulation of the expression and secretion of tumor necrosis factor-alpha (TNF-a) and interleukin-8 (IL-8), and the chemotactic cytokine monocyte chemoattractant protein-1 (MCP-1). The secretory responses were dose-dependent and associated with moderate release of histamine and tryptase, which are preformed mast cell mediators contained in the cytoplasmic secretory granules of the cells. Also native LDL-IgG ICs induced similar pro-inflammatory cytokine response, suggesting that ICs per se are important for the IgG IC-induced mast cell activation. Conclusion Mast cells in atherosclerotic lesions which also contain oxLDL-IgG ICs may become activated by the ICs and secrete many pro-inflammatory cytokines. Our results suggest that intimal mast cells act as a cellular link between oxLDL-IgG ICs and the inflammatory response in atherosclerosis. |
| Databáze: | OpenAIRE |
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