Expression analysis of microRNA-125 in patients with polycythemia vera and essential thrombocythemia and correlation with JAK2 allele burden and laboratory findings
| Autor: | Azita Azarkeivan, Kamran Atarodi, Mohammad Vaezi, Seyed H. Ghaffari, Kamran Alimoghaddam, Reza Shirkoohi, Shirin Ferdowsi, Naser Amirizadeh, Gh. Toogeh, Mohammad Faranoush, H. Teimori Naghadeh, A. Ghavamzadeh |
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| Rok vydání: | 2015 |
| Předmět: |
Male
medicine.medical_specialty Clinical Biochemistry Disease Gastroenterology law.invention Correlation Polycythemia vera Gene Frequency law Internal medicine microRNA medicine Humans Platelet Allele Polycythemia Vera Polymerase chain reaction Alleles Hematologic Tests Essential thrombocythemia business.industry Biochemistry (medical) Hematology General Medicine Janus Kinase 2 medicine.disease MicroRNAs Gene Expression Regulation Case-Control Studies Immunology Mutation Female business Thrombocythemia Essential |
| Zdroj: | International journal of laboratory hematology. 37(5) |
| ISSN: | 1751-553X |
| Popis: | SUMMARY Introduction: The JAK2V617F mutation has emerged in recent years as a diagnostic as well as a treatment target in patients with polycy- themia vera (PV) and essential thrombocythemia (ET). The disease phenotype is also influenced by other factors such as microRNA (miRNA) deregulation. The aim of this study was to investigate miR-125 expression level in these patients with those obtained from healthy control subjects and its correlation with JAK2 allele burden and laboratory findings. Methods: In total, forty patients with a clinical diagnosis of PV and ET were examined at the time of diagnosis. Ten healthy subjects were checked as controls. We performed JAK2 V617F allele burdens mea- surement and expression analysis of miR-125b-5p, miR-125b-3p, miR-125a-5p, and miR-125a-3p in leukocytes isolated from periph- eral blood by quantitative real-time polymerase chain reaction. Results: MiR-125b-5p and miR-125a-5p were upregulated in both patients with PV (P = 0.00 and P = 0.003, respectively) and ET (P = 0.02 and P = 0.002, respectively). In PV group, a significant correlation was observed between miR-125a-5p and platelet counts (P = 0.01, r = 0.531). The correlation between miRNA and JAk2 allele burden was not significant. Conclusion: In conclusion, our data indicate that other factors such as aberrant miR-125 expression may influence on the disease phe- notype in patients with PV and ET. |
| Databáze: | OpenAIRE |
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