Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation
| Autor: | Ana Godinho-Santos, Monika Kapinska-Mrowiecka, Magdalena Król, Michael Dixon, Karin Loré, Luca Cassetta, Derya Karakoc, Verena Müller, Bartłomiej Taciak, Elisabeth Ersvær, Ida Marie Rundgren, Jochen Utikal, Jeffrey W. Pollard, Kirsten Bruderek, Esther D. Kers-Rebel, Thalia Garcia-Tellez, Gunes Esendagli, Utku Horzum, Mark ter Laan, Benedikt Höing, Kerim Bora Yilmaz, Kim C. M. Santegoets, Stephan Lang, Mirjana Gotic, Lubomir Bodnar, Michaela Müller-Trutwin, Christine Bourgeois, Olympia E. Anastasiou, Oktawia Osiecka, Suncica Bjelica, Joanna Skrzeczynska-Moncznik, Viktor Umansky, Sven Brandau, Juan F. Santibanez, Robert Badura, Ang Lin, Joanna Cichy, Gennadiy Zelinskyy, Emilia Górka, Astrid Olsnes Kittang, Ulf Dittmer, Xiaoying Hu, Ana E. Sousa, Gosse J. Adema |
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| Přispěvatelé: | Repositório da Universidade de Lisboa |
| Rok vydání: | 2020 |
| Předmět: |
Male
Cancer Research tumor Myeloid Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2] Immunology Medizin Inflammation Disease 03 medical and health sciences 0302 clinical medicine Immune system Immunophenotyping head and neck neoplasms Neoplasms Immunotherapy Biomarkers medicine Immunology and Allergy Humans immunoassay RC254-282 030304 developmental biology Pharmacology 0303 health sciences business.industry Myeloid-Derived Suppressor Cells Cancer Neoplasms. Tumors. Oncology. Including cancer and carcinogens biomarkers medicine.disease myeloid-derived suppressor cells immunity 3. Good health Reconstructive and regenerative medicine Radboud Institute for Health Sciences [Radboudumc 10] VDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762 Chronic infection medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Myeloid-derived Suppressor Cell Molecular Medicine Female medicine.symptom business cellular |
| Zdroj: | Journal for Immunotherapy of Cancer Journal for ImmunoTherapy of Cancer, Vol 8, Iss 2 (2020) Journal for ImmunoTherapy of Cancer (JITC) e001223 Journal for ImmunoTherapy of Cancer Journal for Immunotherapy of Cancer, 8, 2 8:e001223 Cassetta, L, Bruderek, K, Skrzeczynska-Moncznik, J, Osiecka, O, Hu, X, Rundgren, I M, Lin, A, Santegoets, K, Horzum, U, Godinho-Santos, A, Zelinskyy, G, Garcia-Tellez, T, Bjelica, S, Taciak, B, Olsnes Kittang, A, Hoing, B, Lang, S, Dixon, M, Muller, V, Utikal, J, Karakoc, D, Bora Yilmaz, K, Gorka, E, Bodnar, L, Anastasiou, O, Bourgeois, C, Badura, R, Kapinska-Mrowiecka, M, Gotic, M, ter Laan, M, Kers-Rebel, E, Krol, M, Francisco Santibanez, J, Muller-Trutwin, M, Dittmer, U, Sousa, A E, Esendagli, G, Adema, G, Lore, K, Ersvaer, E, Umansky, V, Pollard, J W, Cichy, J & Brandau, S 2020, ' Differential expansion of circulating human MDSC subsets in patients with cancer, infection and inflammation ', British Medical Journal (BMJ) . https://doi.org/10.1136/jitc-2020-001223 Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Journal for Immunotherapy of Cancer, 8 |
| ISSN: | 2051-1426 |
| DOI: | 10.1136/jitc-2020-001223 |
| Popis: | © Author(s) (or their employer(s)) 2020. Re- use permitted under CC BY- NC. No commercial re- use. See rights and permissions. Published by BMJ. Background: Myeloid-derived suppressor cells (MDSC) are a functional myeloid cell subset that includes myeloid cells with immune suppressive properties. The presence of MDSC has been reported in the peripheral blood of patients with several malignant and non-malignant diseases. So far, direct comparison of MDSC across different diseases and Centers is hindered by technical pitfalls and a lack of standardized methodology. To overcome this issue, we formed a network through the COST Action Mye-EUNITER (www.mye-euniter.eu) with the goal to standardize and facilitate the comparative analysis of human circulating MDSC in cancer, inflammation and infection. In this manuscript, we present the results of the multicenter study Mye-EUNITER MDSC Monitoring Initiative, that involved 13 laboratories and compared circulating MDSC subsets across multiple diseases, using a common protocol for the isolation, identification and characterization of these cells. Methods: We developed, tested, executed and optimized a standard operating procedure for the isolation and immunophenotyping of MDSC using blood from healthy donors. We applied this procedure to the blood of almost 400 patients and controls with different solid tumors and non-malignant diseases. The latter included viral infections such as HIV and hepatitis B virus, but also psoriasis and cardiovascular disorders. Results: We observed that the frequency of MDSC in healthy donors varied substantially between centers and was influenced by technical aspects such as the anticoagulant and separation method used. Expansion of polymorphonuclear (PMN)-MDSC exceeded the expansion of monocytic MDSC (M-MDSC) in five out of six solid tumors. PMN-MDSC expansion was more pronounced in cancer compared with infection and inflammation. Programmed death-ligand 1 was primarily expressed in M-MDSC and e-MDSC and was not upregulated as a consequence of disease. LOX-1 expression was confined to PMN-MDSC. Conclusions: This study provides improved technical protocols and workflows for the multi-center analysis of circulating human MDSC subsets. Application of these workflows revealed a predominant expansion of PMN-MDSC in solid tumors that exceeds expansion in chronic infection and inflammation. Lab10: This work was funded by the following grants: PTDC/MED- IMU/30474/2017 – project cofunded by FEDER LISBOA-01–0145- FEDER-030474, through Programa Operacional Regional de Lisboa, do PORTUGAL 2020, and Fundação para a Ciência e a Tecnologia and by Gilead Genesis to AES. |
| Databáze: | OpenAIRE |
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