Cytotoxic effects of dental calculus particles and freeze-dried Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum on HSC-2 oral epithelial cells and THP-1 macrophages
| Autor: | Yukio Ozaki, Atsutoshi Yoshimura, Kanako Higuchi, Yasunori Yamashita, S.M. Ziauddin, Mohammad Ibtehaz Alam, Masayuki Oohira, Megumi Mae |
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| Rok vydání: | 2021 |
| Předmět: |
biology
Fusobacterium nucleatum Chemistry Calculus (dental) Macrophages Pyroptosis Aggregatibacter actinomycetemcomitans Epithelial Cells biology.organism_classification Dental plaque medicine.disease Microbiology Dental deposit Squamous carcinoma stomatognathic diseases stomatognathic system Glyburide medicine Periodontics Cytotoxic T cell Humans Dental Calculus Porphyromonas gingivalis |
| Zdroj: | Journal of periodontologyREFERENCES. 93(6) |
| ISSN: | 1943-3670 |
| Popis: | Background Periodontitis is an inflammatory disease initiated by dental deposits. Microorganisms in the dental biofilm induce cell death in epithelial cells, contributing to the breakdown of epithelial barrier function. Recently, dental calculus has also been implicated in pyroptotic cell death in oral epithelium. We analyzed the cytotoxic effects of dental calculus and freeze-dried periodontopathic bacteria on oral epithelial cells and macrophages. Methods HSC-2 human oral squamous carcinoma cells and PMA-differentiated THP-1 macrophages were exposed to dental calculus or one of two species of freeze-dried bacterium, Aggregatibacter actinomycetemcomitans and Fusobacterium nucleatum. Following incubation for 24 h, we measured cytotoxicity via lactate dehydrogenase release. Cells were then incubated with glyburide, an NLRP3 inflammasome inhibitor, to assess the potential role of pyroptosis. We also conducted a permeability assay to analyze the effects on epithelial barrier function. Results Dental calculus induced dose-dependent cell death in HSC-2 cells, whereas cell death induced by freeze-dried bacteria was insignificant. Conversely, freeze-dried bacteria induced more cell death than dental calculus in THP-1 macrophages. Cell death induced by dental calculus but not by freeze-dried bacteria was inhibited by glyburide, indicating that these are different types of cell death. In the permeability assays, dental calculus but not freeze-dried bacteria attenuated the barrier function of HSC-2 cell monolayers. Conclusion Due to the low sensitivity of HSC-2 cells to microbial cytotoxicity, dental calculus had stronger cytotoxic effects on HSC-2 cell monolayers than freeze-dried A. actinomycetemcomitans and F. nucleatum, suggesting that it plays a critical role in the breakdown of crevicular/pocket epithelium integrity. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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