Novel insights into DNA methylation features in spermatozoa: stability and peculiarities

Autor: Juan Sandoval, Chiara Chianese, Claudia Giachini, Holger Heyn, Manel Esteller, Sergi Sayols, Csilla Krausz
Rok vydání: 2012
Předmět:
Male
ADN
Biochemistry
Epigenesis
Genetic
chemistry.chemical_compound
0302 clinical medicine
Databases
Genetic
Molecular Cell Biology
Genetics
0303 health sciences
030219 obstetrics & reproductive medicine
Multidisciplinary
Methylation
Middle Aged
Spermatozoa
Nucleic acids
Espermatozoides
Vocabulary
Controlled
CpG site
DNA methylation
Medicine
Epigenetics
Cellular Types
DNA modification
Research Article
Adult
Urology
Science
Cèl·lules B
Biophysics
Biology
Adult
CpG Islands
DNA Methylation
Databases
Genetic
Epigenesis
Genetic
Humans
Male
Middle Aged
RNA
Small Untranslated
metabolism
Spermatozoa
metabolism
Vocabulary
Controlled
03 medical and health sciences
Humans
Gene
030304 developmental biology
B cells
Cell swimming
urogenital system
DNA
DNA Methylation
Epigenètica
Sperm
Germ Cells
chemistry
Infertility
RNA
Small Untranslated
CpG Islands
Gene expression
Zdroj: Dipòsit Digital de la UB
Universidad de Barcelona
PLoS ONE, Vol 7, Iss 10, p e44479 (2012)
Plos One
r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe
instname
PLoS One
r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
Universitat Politècnica de Catalunya (UPC)
PLoS ONE
Recercat. Dipósit de la Recerca de Catalunya
ISSN: 1932-6203
Popis: Data about the entire sperm DNA methylome are limited to two sperm donors whereas studies dealing with a greater number of subjects focused only on a few genes or were based on low resolution arrays. This implies that information about what we can consider as a normal sperm DNA methylome and whether it is stable among different normozoospermic individuals is still missing. The definition of the DNA methylation profile of normozoospermic men, the entity of inter-individual variability and the epigenetic characterization of quality-fractioned sperm subpopulations in the same subject (intra-individual variability) are relevant for a better understanding of pathological conditions. We addressed these questions by using the high resolution Infinium 450K methylation array and compared normal sperm DNA methylomes against somatic and cancer cells. Our study, based on the largest number of subjects (n = 8) ever considered for such a large number of CpGs (n = 487,517), provided clear evidence for i) a highly conserved DNA methylation profile among normozoospermic subjects; ii) a stable sperm DNA methylation pattern in different quality-fractioned sperm populations of the same individual. The latter finding is particularly relevant if we consider that different quality fractioned sperm subpopulations show differences in their structural features, metabolic and genomic profiles. We demonstrate, for the first time, that DNA methylation in normozoospermic men remains highly uniform regardless the quality of sperm subpopulations. In addition, our analysis provided both confirmatory and novel data concerning the sperm DNA methylome, including its peculiar features in respect to somatic and cancer cells. Our description about a highly polarized sperm DNA methylation profile, the clearly distinct genomic and functional organization of hypo- versus hypermethylated loci as well as the association of histone-enriched hypomethylated loci with embryonic development, which we now extended also to hypomethylated piRNAs-linked genes, provides solid basis for future basic and clinical research.
Databáze: OpenAIRE
Externí odkaz: