Transduction of murine cerebellar neurons with recombinant FIV and AAV5 vectors
Autor: | Douglas J. Jolly, John A. Chiorini, Sybille L. Sauter, Stephanie M. Hughes, Thomas W. Dubensky, Joseph Martin Alisky, Beverly L. Davidson, Patrick D. Staber |
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Rok vydání: | 2000 |
Předmět: |
Cerebellum
Feline immunodeficiency virus Genetic Vectors Central nervous system Biological Transport Active Immunodeficiency Virus Feline Deep cerebellar nuclei Recombinant virus Mice Purkinje Cells Transduction (genetics) Vestibular nuclei Transduction Genetic medicine Animals Transgenes Neurons biology General Neuroscience Dependovirus beta-Galactosidase biology.organism_classification Mice Inbred C57BL medicine.anatomical_structure nervous system Axoplasmic transport Neuroscience |
Zdroj: | NeuroReport. 11:2669-2673 |
ISSN: | 0959-4965 |
Popis: | Our data demonstrate that vectors derived from recombinant feline immunodeficiency virus (rFIV) and adeno-associated virus type 5 (rAAV5) transduce cerebellar cells following direct injection into the cerebellar lobules of mice. Both recombinant viruses mediated gene transfer predominantly to neurons, with up to 2500 and 1500 Purkinje cells transduced for rAAV5 or rFIV-based vectors, respectively. The vectors also transduced stellate, basket and Golgi neurons, with occasional transduction of granule cells and deep cerebellar nuclei. rAAV5 also spread outside the cerebellum to the inferior colliculus and ventricular epithelium, while rFIV demonstrated the ability to undergo retrograde transport to the physically close lateral vestibular nuclei. Thus, AAV5 and FIV-based vectors show promise for targeting neurons affected in the hereditary spinocerebellar ataxias. These vectors could be important tools for unraveling the pathophysiology of these disorders, or in testing factors which may promote neuronal survival. |
Databáze: | OpenAIRE |
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