Biosynthesis of poly-3-hydroxybutyrate from grass silage by a two-stage fermentation process based on an integrated biorefinery concept
| Autor: | Dominik Schwarz, Cordt Zollfrank, Jan Sterba, Josef Vilímek, Pavla Fojtíková, Doris Schieder, Amelie K.J. Schoenenwald, Volker Sieber, David Kahoun, Jörg Dörrstein |
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| Přispěvatelé: | Publica |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Environmental Engineering Silage Polyesters Cupriavidus necator 030106 microbiology Hydroxybutyrates Bioengineering Bacterial growth Xylose Poaceae 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Bioreactors 010608 biotechnology Press cake Food science Waste Management and Disposal biology Renewable Energy Sustainability and the Environment Chemistry food and beverages General Medicine Biorefinery biology.organism_classification Fermentation Lactic acid fermentation |
| Popis: | Grass silage as a renewable feedstock for an integrated biorefinery includes nutrients and carbon sources directly available in the press juice (PJ) and in lignocellulosic saccharides from the plant framework. Here, a novel two-stage fed-batch fermentation process for biosynthesis of poly-3-hydroxybutyrate (PHB) by Cupriavidus necator DSM 531 is presented. For bacterial growth, nutrient-rich PJ was employed as a fermentation medium, without any supplements. Saccharides derived from the mechano-enzymatic hydrolysis of the press cake (PC) were subjected to a lactic acid fermentation process, before the fermentation products were fed into the polymer accumulation phase. By combination of pH-stat feeding and cell recycling, the PHB content in 22 g L−1 total-dry cells reached 39% after 32 h of cultivation. Using mimicked hydrolyzate of diluted PJ artificially supplemented with glucose and xylose, the resulting cell dry weight of 21 g L−1 contained 42% PHB. |
| Databáze: | OpenAIRE |
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