Steady-State Electrochemiluminescence at Single Semiconductive Titanium Dioxide Nanoparticles for Local Sensing of Single Cells
| Autor: | Jian-Rong Zhang, Jun-Jie Zhu, Dechen Jiang, Chen Cui, Ying Chen, Hong-Yuan Chen |
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| Rok vydání: | 2018 |
| Předmět: |
endocrine system
Hydrogen Nanoparticle chemistry.chemical_element 010402 general chemistry Photochemistry Electrochemistry 01 natural sciences Analytical Chemistry chemistry.chemical_compound Adsorption Electrochemiluminescence Humans Hydrogen peroxide Titanium Molecular Structure Chemistry 010401 analytical chemistry Optical Imaging Electrochemical Techniques 0104 chemical sciences Semiconductors Rutile Titanium dioxide Luminescent Measurements Nanoparticles Single-Cell Analysis HeLa Cells |
| Zdroj: | Analytical chemistry. 91(1) |
| ISSN: | 1520-6882 |
| Popis: | Obtaining steady-state bright electrochemiluminescence (ECL) at single nanoparticles is crucial but challenging for the realization of the single-nanoparticle electrochemical sensing of single cells. In this work, steady-state bright ECL is implemented at single semiconductive titanium dioxide (TiO2) nanoparticles for sensing the local efflux from single living cells. Oxygen vacancies on the surface of rutile TiO2 nanoparticles have a high affinity for hydrogen peroxides that are not easily passivated upon exposure to voltage. Therefore, the steady-state adsorption of hydrogen peroxide by the TiO2 nanoparticle surface permits the continuous electrochemical generation of superoxide and hydroxyl radicals by electrons and surface-trapped holes at the nanoparticles, resulting in constant ECL under physiological conditions. This steady-state luminescence during continuous imaging is correlated with the concentration of hydrogen peroxide, leading to the local ECL visualization of hydrogen peroxide efflux from s... |
| Databáze: | OpenAIRE |
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