Comparative Genomics of the IncA/C Multidrug Resistance Plasmid Family
| Autor: | J. Eugene LeClerc, Jacques Ravel, David G. White, Mark K. Mammel, Patrick F. McDermott, Timothy J. Welch, Thomas A. Cebula, W. Florian Fricke |
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| Rok vydání: | 2009 |
| Předmět: |
Genomics and Proteomics
Sequence analysis Molecular Sequence Data Biology medicine.disease_cause Microbiology Plasmid maintenance Plasmid Drug Resistance Multiple Bacterial Host chromosome Gene cluster Escherichia coli medicine Molecular Biology Gene Phylogeny Genetics Models Genetic Genomics Sequence Analysis DNA Aeromonas hydrophila Multiple drug resistance Algorithms Plasmids |
| Zdroj: | Journal of Bacteriology. 191:4750-4757 |
| ISSN: | 1098-5530 0021-9193 |
| Popis: | Multidrug resistance (MDR) plasmids belonging to the IncA/C plasmid family are widely distributed among Salmonella and other enterobacterial isolates from agricultural sources and have, at least once, also been identified in a drug-resistant Yersinia pestis isolate (IP275) from Madagascar. Here, we present the complete plasmid sequences of the IncA/C reference plasmid pRA1 (143,963 bp), isolated in 1971 from the fish pathogen Aeromonas hydrophila , and of the cryptic IncA/C plasmid pRAx (49,763 bp), isolated from Escherichia coli transconjugant D7-3, which was obtained through pRA1 transfer in 1980. Using comparative sequence analysis of pRA1 and pRAx with recent members of the IncA/C plasmid family, we show that both plasmids provide novel insights into the evolution of the IncA/C MDR plasmid family and the minimal machinery necessary for stable IncA/C plasmid maintenance. Our results indicate that recent members of the IncA/C plasmid family evolved from a common ancestor, similar in composition to pRA1, through stepwise integration of horizontally acquired resistance gene arrays into a conserved plasmid backbone. Phylogenetic comparisons predict type IV secretion-like conjugative transfer operons encoded on the shared plasmid backbones to be closely related to a group of integrating conjugative elements, which use conjugative transfer for horizontal propagation but stably integrate into the host chromosome during vegetative growth. A hipAB toxin-antitoxin gene cluster found on pRA1, which in Escherichia coli is involved in the formation of persister cell subpopulations, suggests persistence as an early broad-spectrum antimicrobial resistance mechanism in the evolution of IncA/C resistance plasmids. |
| Databáze: | OpenAIRE |
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