Analysis of the interaction of procathepsin D activation peptide with breast cancer cells
| Autor: | Vaclav Vetvicka, Jana Vetvickova, Zdenek Voburka, Martin Fusek, Ivan Hilgert |
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| Rok vydání: | 1997 |
| Předmět: |
Cancer Research
Cathepsin D Mice Nude Breast Neoplasms Biology medicine.disease_cause Antibodies Receptor IGF Type 2 Flow cytometry Cell Line Mice Cell surface receptor medicine Animals Humans Secretion Receptors Growth Factor Receptor Fluorescent Dyes Enzyme Precursors Mice Inbred BALB C medicine.diagnostic_test Cancer medicine.disease Molecular biology Enzyme Activation Oncology Biochemistry Cancer cell Carcinogenesis Fluorescein-5-isothiocyanate Neoplasm Transplantation |
| Zdroj: | International journal of cancer. 73(3) |
| ISSN: | 0020-7136 |
| Popis: | Cathepsin D, a lysosomal aspartic proteinase, is secreted in the form of enzymatically inactive proenzyme by many types of human breast cancer tissue and exerts mitogenic activity toward these tissues. Flow cytometry was used to test the binding of procathepsin D purified from the secretion of the breast cancer cell line ZR-75-1 to human breast cancer cells. No previously known surface antigens or soluble M6P-R or anti-M6P-R antibodies were found to inhibit the specific binding of procathepsin D-FITC. Similarly, none of these potential inhibitors was found to inhibit growth factor activity of procathepsin D. Our results indicate that procathepsin D growth factor activity is mediated by a new, previously unknown receptor moiety and that the binding activity can be localized in position 27-44 of the activation peptide of procathepsin D. Furthermore, in vivo experiments indicate that treatment with anti-procathepsin D antibodies can reverse the growth of human breast tumors in athymic nude mice. |
| Databáze: | OpenAIRE |
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